H_(2)O_(2)mediates transcriptome reprogramming during Soybean mosaic virus-induced callose deposition in soybean  

作　　者：Tianjie Sun Xizhe Sun Fukuan Li Nan Ma Mengxuan Wang Yan Chen Na Liu Yuan Jin Jie Zhang Chunyan Hou Chunyan Yang Dongmei Wang 

机构地区：[1]State Key Laboratory of North China Crop Improvement and Regulation,Baoding 071000,Hebei,China [2]Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology,Baoding 071000,Hebei,China [3]College of Life Sciences,Hebei Agricultural University,Baoding 071000,Hebei,China [4]Market Supervision Administration of Chongli District,Zhangjiakou 076350,Hebei,China [5]Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050035,Hebei,China

出　　处：《The Crop Journal》2022年第1期262-272,共11页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(30971706 and31471421);"973"Preliminary Program(2014CB160318);Hebei Natural Science Foundation(C2020204132)。

摘　　要：The main defense response to Soybean mosaic virus(SMV)infection in soybean[Glycine max(L.)Merr.]is thought to be blockage of intercellular virus transport by callose deposition on plasmodesmata.But the specific regulatory mechanism remains largely unknown.In this study,we found that hydrogen peroxide(H_(2)O_(2))signal downstream of NO was associated with the regulation of callose accumulation.Abundant H_(2)O_(2)was produced on the cell membrane and cell wall in the incompatible combination of soybean cultivar Jidou 7 and SMV strain N3,whereas no obvious H_(2)O_(2)was observed in the compatible combination of Jidou 7 and strain SC-8.When H_(2)O_(2)production was inhibited,callose accumulation induced by SMV infection decreased to a level insufficient to restrict virus transport in the incompatible combination.The H_(2)O_(2)-associated transcriptome dynamics of soybean during SMV infection was investigated.Transcriptome and functional analysis using virus-induced gene silencing showed that Gm SEOB and Gm PAP27,two genes regulated by H_(2)O_(2),functioned in resistance by positively regulating the accumulation of callose in response to SMV infection.These results lay a foundation for further research on the signal transduction and molecular regulation of callose deposition during soybean resistance to SMV infection.

关 键 词：CALLOSE Soybean mosaic virus H_(2)O_(2) TRANSCRIPTOME SOYBEAN 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]