GmNMHC5 may promote nodulation via interaction with GmGAI in soybean  

作　　者：Wenting Wang Zhili Wang Wensheng Hou Li Chen Bingjun Jiang Wenya Ma Lijuan Bai Wenwen Song Cailong Xu Tianfu Han Yongjun Feng Cunxiang Wu 

机构地区：[1]School of Life Science,Beijing Institute of Technology,Beijing 100081,China [2]MARA Key Laboratory of Soybean Biology(Beijing),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《The Crop Journal》2022年第1期273-279,共7页作物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31271636);the China Agriculture Research System(CARS-04)。

摘　　要：Soybean(Glycine max[L.]Merr.)is a food and oil crop whose growth and yield are influenced by root and nodule development.In the present study,Gm NMHC5 was found to promote the formation of nodules in overexpressing mutants.In contrast,the number of nodules in Gmnmhc5 edited with CRISPR/Cas9 decreased sharply.In 35 S:Gm NMHC5 mutants,expression levels of genes involved in nodulation were significantly up-regulated.Both in vitro and in vivo biochemical analyses showed that Gm NMHC5 directly interacted with Gm GAI(a DELLA protein),and the content of gibberellin 3(GA_(3))in overexpressing mutants was lower than that in the wild type.These results revealed that Gm NMHC5 participates in the classical GA signaling pathway,and may regulate the content of GA_3 to match the optimal concentration required for nodule formation,thereby promoting nodulation by directly interacting with Gm GAI.A model illustrating the mechanism by which Gm NMHC5 promotes soybean nodulation is presented.

关 键 词：Glycine max GmNMHC5 GmGAI NODULATION GA pathway 

分 类 号：S565.1[农业科学—作物学]