染料木素通过调节能量代谢和抑制氧化应激缓解大鼠运动性疲劳的研究  

Genistein in alleviating rat sports fatigue by regulating energy metabolism and inhibiting oxidative stress

作　　者：麻海娟 曾雪亮[1,3] 李贝 黎晓[1,2] 吴丽珍[1,2] 李良东[1,3] 黄诚[1,2] 黄志华[1,2] MA Hai-juan;ZENG Xue-liang;LI Bei;LI Xiao;WU Li-zhen;LI Liang-dong;HUANG Cheng;HUANG Zhi-hua(Key Laboratory of Ministry of Education for Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases,Gannan Medical University;Pain Medicine Research Institute,Gannan Medical University;The First Affiliated Hospital of Gannan Medical University,Ganzhou,Jiangxi 341000)

机构地区：[1]赣南医学院心脑血管疾病防治教育部重点实验室 [2]赣南医学院疼痛医学研究所 [3]赣南医学院第一附属医院,江西赣州341000

出　　处：《赣南医学院学报》2021年第12期1193-1202,共10页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：National Natural Science Foundation of China(No. 31360250)。

摘　　要：目的:染料木素(Genistein,Gen)对运动性疲劳的潜在作用及其机制。方法:SD雄性大鼠随机分为4组,通过游泳运动构建运动性疲劳大鼠模型。游泳前30 min,模型组、对照组皮下注射生理盐水,给药组分别皮下注射0.1μmol·kg^(-1)和0.3μmol·kg^(-1)的Gen,对照组不进行游泳运动,模型组、给药组被迫游泳,每天1次,连续游泳14 d。最后一次游泳后,测定各组的血糖、肌糖原、肝糖原、T-AOC、GSH-Px、SOD、MDA水平,肌肉和肝脏Na^(+)-K^(+)-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶活性,以及股四头肌中的LDH和CK。结果:Gen可提高运动疲劳大鼠的血糖、肌糖原和肝糖原水平,同时升高血清T-AOC、GSH-PX、SOD,降低血清MDA。此外,还可增加肌肉和肝脏中Na^(+)-K^(+)-ATP酶、Ca^(2+)-Mg^(2+)-ATP酶、LDH和CK的活性。结论:Gen可有效地缓解大鼠的运动疲劳,其机制可能与调节能量代谢、维持自由基和抗氧化系统平衡有关。Objective:To evaluate the potential beneficial effects and the underlying mechanism of Gen on the sports fatigue.Methods:Male SD rats were randomly and uniformly divided into four groups(control,vehicle,0. 1 μmol·kg^(-1) Gen,and 0. 3 μmol·kg^(-1) Gen),and sports fatigue was induced by swimming exercise. Thirty minutes before swimming,normal saline was injected subcutaneously into the control group and vehicle group. The treatment group was subcutaneously injected with 0. 1 μmol·kg^(-1) Gen,and 0. 3 μmol·kg^(-1) Gen,The control group was not subjected to swimming exercise.The vehicle group and the treatment group were forced to swim once a day for 14 days. The potential anti-fatigue functions induced by Gen,and the underlying mechanism were evaluated after the last exhaustive swimming by determining the levels of blood glucose,muscle glycogen,hepatic glycogen,Total antioxidant capacity(T-AOC),Glutathione peroxidase(GSH-Px),Superoxide dismutase(SOD),and Malondialdehyde(MDA). The determination was done about the activities of Na^(+)-K^(+)-ATPase and Ca^(2+)-Mg^(2+)-ATPase in both muscle and liver,and the activities of lactate dehydrogenase(LDH)and creatine kinase(CK)in the quadriceps femoris muscle.Results :Gen increased the consumption of blood glucose as well as the levels of muscle glycogen and hepatic glycogen. It also increased the serum T-AOC,GSH-PX and SOD but decreased the serum MDA. In addition,gen increased the activities of Na^(+)-K^(+)-ATPase,Ca^(2+)-Mg^(2+)-ATPase,LDH and CK.Conclusion:Gen could protect against sports fatigue by regulating energy metabolism and maintaining the free radical and antioxidant system balance.

关 键 词：染料木素 运动疲劳 能量代谢 抗氧化系统 

分 类 号：R873.1[医药卫生—运动医学]