局部淋巴结试验与局部封闭斑贴试验应用于化妆品皮肤变态反应评价的比较  

Comparision of the Application of the Local Lymph Node Assay and Buehler Test in Evaluation of Skin Sensitization for Cosmetics

作　　者：桑晶 段名扬 陈舒怀 蒙宏 孙叶丹 朱社敏 匡荣 SANG Jing;DUAN Mingyang;CHEN Shuhuai;MENG Hong;SUN Yedan;ZHU Shemin;KUANG Rong(Zhejiang Institute for Food and Drug Control,NMPA Key Laboratory for Animal Alternative Testing Technology of Cosmetics,Hangzhou 310052,China;Collaboration Innovation Center of Green Pharmaceuticals,Zhejiang University of Technology,Hangzhou 310014,China)

机构地区：[1]浙江省食品药品检验研究院,国家药品监督管理局化妆品动物替代试验技术重点实验室,杭州310052 [2]浙江工业大学绿色制药协同中心,杭州310014

出　　处：《中国现代应用药学》2021年第24期3078-3081,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：浙江省药品监管系统科技计划项目(2020010)。

摘　　要：目的通过局部淋巴结试验法和局部封闭斑贴试验应用于化妆品皮肤变态反应的比较,探讨局部淋巴结试验应用于化妆品成品的可行性。方法采用局部淋巴结试验与体内局部封闭斑贴试验对标准的阳性物以及市面上的2种染发剂进行评价。结果局部淋巴结试验表明,2种染发剂的SI值<1.8,均为阴性,判断结果与局部封闭斑贴试验结果一致。结论局部淋巴结试验相对局部封闭斑贴试验具有检验周期短、测试简便、评价客观等优点,是一种较好的用于评价染发类态产品过敏反应的替代方法。OBJECTIVE To explore the possibility of local lymph node test(LLNA)in evaluation for cosmetic products by compare the application of LLNA and Buehler test in evaluation of skin sensitization for cosmetics.METHODS LLNA and Buehler test in vivo were used to evaluate the standard positive control and two kinds of hair dyes on the market.RESULTS The result of the LLNA showed the SI data of two hair dyes were below 1.8 with negative result,which were consistent with the result of Buehler test.CONCLUSION Compared with the Buehler test,the LLNA has the advantages of short test cycle,test simple and objective evaluation,it is a good alternative method for evaluating skin sensitization of hair dyeing products.

关 键 词：局部淋巴结试验 皮肤变态反应 替代方法 

分 类 号：R965.1[医药卫生—药理学]