HPLC-超滤法测定羟基酪醇与常氧及缺氧大鼠血浆蛋白的结合率  

Determination of Plasma Protein Binding Rate of Hydroxytyrosol in Normoxic and Hypoxic Rat by HPLCUltrafiltration Method

作　　者：王维刚 王芃 李晓琳 刘天龙 杜玮泽 郭姿良 陈升富 李茂星 WANG Weigang;WANG Peng;LI Xiaolin;LIU Tianlong;DU Weize;GUO Ziliang;CHEN Shengfu;LI Maoxing(School of Pharmacy,Gansu University of Chinese Medicine,Lanzhou 730000,China;Department of Clinical Pharmacy,The 940th Hospital of Joint Logistic Support Force of PLA,Lanzhou 730050,China;Gansu Plateau Pharmaceutical Technology Center,Lanzhou 730050,China;School of Pharmacy,Lanzhou University,Lanzhou 730030,China)

机构地区：[1]甘肃中医药大学药学院,兰州730000 [2]联勤保障部队第九四〇医院临床药学科,兰州730050 [3]甘肃省高原药学行业技术中心,兰州730050 [4]兰州大学药学院,兰州730030

出　　处：《中国现代应用药学》2021年第24期3167-3172,共6页Chinese Journal of Modern Applied Pharmacy

基　　金：甘肃省重点研发计划项目(20YF3FA035);中央高校基本科研业务费专项资金项目(31920200010);940医院青年培育项目(2021yxky060);940医院应急医学科研项目(20yjky018)。

摘　　要：目的建立羟基酪醇血浆蛋白结合率的测定方法,比较常氧及缺氧大鼠血浆中羟基酪醇蛋白结合率的异同。方法采用HPLC-超滤法测定羟基酪醇与常氧及缺氧大鼠血浆的蛋白结合率。结果当大鼠血浆中羟基酪醇浓度为3,6,12μg·mL^(–1)时,羟基酪醇与常氧大鼠血浆的蛋白结合率分别为(17.23±1.11)%,(16.88±1.37)%和(16.70±0.98)%,与缺氧大鼠血浆的蛋白结合率分别为(12.31±1.79)%,(12.75±1.20)%和(13.42±1.98)%,两者比较差异有统计学意义(P<0.01)。结论羟基酪醇与常氧大鼠血浆蛋白属于低强度结合,且在3~12μg·mL^(–1)内,不具浓度依赖性,而羟基酪醇与缺氧大鼠血浆蛋白结合强度明显低于与常氧大鼠血浆蛋白的结合。OBJECTIVE To establish a method for determination of plasma protein binding rate of hydroxytyrosol,and compare the difference of plasma protein binding rate in normoxic and hypoxic rats.METHODS The protein binding rate of hydroxytyrosol in normoxic and hypoxic rats plasma was determined by HPLC-ultrafiltration.RESULTS When the concentration of hydroxytyrosol in rat plasma was 3,6 and 12μg·mL^(-1),the plasma protein binding rates of hydroxytyrosol with normoxic rats were(17.23±1.11)%,(16.88±1.37)%and(16.70±0.98)%,and the plasma protein binding rates with hypoxic rats were(12.31±1.79)%,(12.75±1.20)%and(13.42±1.98)%.The difference of the plasma protein binding rate was significant between the normoxic and hypoxic rats(P<0.01).CONCLUSION The binding strength of hydroxytyrosol to plasma protein of normoxic rats is low and does not show concentration dependence within the concentration range of 3–12μg·mL^(-1).The binding strength of hydroxytyrosol to plasma protein of hypoxic rats is significantly lower than that of hydroxytyrosol to plasma protein of normoxic rats.

关 键 词：羟基酪醇 HPLC-超滤法 常氧 缺氧 血浆蛋白结合率 

分 类 号：R917.101[医药卫生—药物分析学]