柱前衍生化HPLC测定复方谷氨酰胺肠溶胶囊中的L-谷氨酰胺  

Determination of L-Glutamine in Compound Glutamine Entresoluble Capsules by Pre-column Derivatization HPLC

作　　者：谷亦平 杨园 GU Yiping;YANG Yuan(School of Public Health,Guilin Medical University,Guilin 541199,China;Wuhan Institute for Drug and Medical Device Control,Wuhan 430075,China;The Guangxi Key Laboratory of Environmental Exposomics and Entire Lifecycle Health,Guilin 541199,China)

机构地区：[1]桂林医学院公共卫生学院,广西桂林541199 [2]武汉药品医疗器械检验所,武汉430075 [3]广西环境暴露组学与全生命周期健康重点实验室,广西桂林541199

出　　处：《中国现代应用药学》2022年第1期78-81,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：湖北省食品药品监督管理局科研项目(201601017)。

摘　　要：目的建立柱前衍生化HPLC测定复方谷氨酰胺肠溶胶囊中L-谷氨酰胺的含量。方法采用CAPCELL PAK C_(18)色谱柱(150 mm×4.6 mm,5μm),以乙酸钠缓冲液(pH 6.4)-乙腈为流动相,梯度洗脱,检测波长为254 nm。以牛磺酸为内标物质,异硫氰酸苯酯为衍生剂,采用单因素和正交试验确定衍生反应最佳条件。结果L-谷氨酰胺在0.0159~0.143 mg·mL^(–1)内线性关系良好(r^(2)=0.9999),加样回收率为100.3%,RSD为0.8%(n=9)。结论本方法准确、重复性好,可用于复方谷氨酰胺肠溶胶囊中L-谷氨酰胺的含量测定。OBJECTIVE To establish a pre-column derivatization HPLC for determination of L-glutamine in Compound Glutamine Entresoluble capsules.METHODS HPLC experiment was performed on a CAPCELL PAK C_(18) column(150 mm×4.6 mm,5μm).The mobile phase were sodium acetate buffer solution(pH 6.4)and acetonitrile with gradient elution.The detection wavelength was set at 254 nm.Taurine was used as an internal standard substance,the sample and taurine were derivatized with phenyl isothiocyanate.The combination of single factor and orthogonal experiments were used to optimize the derivatization experiment conditions.RESULTS The L-glutamine had good linearity in the range of 0.0159−0.143 mg·mL^(–1)(r^(2)=0.9999).The average recoveries was 100.3%with RSD of 0.8%(n=9).CONCLUSION The HPLC method is accruate with high repeatability,which is helpful for the determination of L-glutamine in Compound Glutamine Entresoluble capsules.

关 键 词：复方谷氨酰胺肠溶胶囊 L-谷氨酰胺 柱前衍生 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]