琥珀酸曲格列汀对2型糖尿病小鼠肠道菌群的改变  

Trigliptin succinate have an effect on gut microbiota of type 2 diabetic mice

作　　者：陈倩 王亚峰[2] 朵德龙[2] 常亚娥 严英俊 段坤坤 CHEN Qian;WANG Yafeng;DUO Delong;CHANG Ya'e;YAN Yingjun;DUAN Kunkun(Qinghai University Medical College,Xining 810000,Qinghai,China;Department of Pharmacy,Qinghai Provincial People's Hospital,Xining 810000,Qinghai,China)

机构地区：[1]青海大学医学院,青海西宁810000 [2]青海省人民医院药学部,青海西宁810007

出　　处：《中国临床药理学与治疗学》2022年第1期15-24,共10页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：青海省科技厅科技成果转化专项项目(2020-SF-131)。

摘　　要：目的:研究琥珀酸曲格列汀对2型糖尿病小鼠肠道菌群的影响。方法:16S rRNA高通量测序法对健康组、T2DM组、琥珀酸曲格列汀组、磷酸西格列汀组小鼠肠道菌群进行测序,利用QIME对数据进行过滤,对物种进行分类和注释。对样品的Alpha多样性指数和Beta多样性指数进行了分析,比较4组的样本菌群丰富度和多样性。结果:结果表明健康组、T2DM组、琥珀酸曲格列汀组、磷酸西格列汀组小鼠肠道菌群结构差异具有统计学意义。与健康组相比,T2DM组的厚壁菌门与拟杆菌门的比值降低,与琥珀酸曲格列汀组比较,T2DM组的Cyanobacteria、Verrucomicrobia、Tenericutes差异具有统计学意义(P<0.05);T2DM组候选生物标志物可能是:Bacilli、Lactobacillales、Lactococcus、Streptococcaceae;琥珀酸曲格列汀组候选生物标志物可能是:Bacteroidia、Bacteroidetes、Bacteroidales、Prevotella、Paraprevotellaceae、Parabacteroides、Porphyromonadaceae;磷酸西格列汀组候选生物标志物可能是:Lactobacillus、Lactobacillaceae、Helicobacter。结论:使用琥珀酸曲格列汀改善小鼠肠道菌落,可能通过改善肠道菌群而达到降糖效果。AIM: To study the effects of trigliptin succinate on gut microbiota of type 2 diabetic mice. METHODS: 16 S rRNA high-throughput sequencing method was used to sequence the intestinal flora of mice in the healthy group, the T2 DM group, the trigliptin succinate group and the sitagliptin phosphate group. QIME was used to filter the data, classify and annotate the species. Alpha diversity index and Beta diversity index of the samples were analyzed.The richness and diversity of bacteria in the four groups were compared. RESULTS: The gut microbiota structure of mice in the healthy group, the T2DM group, the trigliptin succinate group and the setagliptin phosphate group were significantly different. The results showed that the ratio of Firmicutes to Bacteroidetes was decreased compared with that in the healthy group. Cyanobacteria, Verrucomicrobia and Tenericutes had significant differences(P<0.05). Potential biomarkers for T2DM group were Bacilli, Lactobacillales, Lactococcus and Streptococcaceae. Candidate biomarkers of trigliptin succinate group may be Bacteroidia, Bacteroidetes, Bacteroidales, Prevotella, Paraprevotellaceae, Parabacteroides, Porphyromonadaceae;The candidate biomarkers of sitagliptin phosphate group may be Lactobacillus, Lactobacillaceae and Helicobacter. CONCLUSION: The intestinal flora of mice in the trigliptin succinate group was significantly different from that in the healthy group and the T2DM group. Using trigliptin succinate to improve the intestinal flora of mice might achieve the hypoglycemic effect by improving the intestinal flora.

关 键 词：2型糖尿病 肠道菌群 琥珀酸曲格列汀 16S rRNA 

分 类 号：R965.2[医药卫生—药理学]