复方芩兰口服液调控流感病毒感染致重症肺炎动物模型免疫功能的机制研究  

The Mechanism of Compound Qinlan Oral Liquid in Regulating Immune Function in Animal Model of Severe Pneumonia Caused by Influenza Virus Infection

作　　者：郭清[1] 王龙[1] 黄猛[1] 李允 GUO Qing;WANG Long;HUANG Meng;LI Yun(Department of Respiratory and Critical Care Medicine,Gaozhou City People's Hospital,Gaozhou 525200,China)

机构地区：[1]高州市人民医院呼吸与危重症医学科,广东高州525200

出　　处：《西部中医药》2021年第12期20-24,共5页Western Journal of Traditional Chinese Medicine

摘　　要：目的:探究复方芩兰口服液通过NF-κB通路对流感病毒感染致重症肺炎(severe pneumonia,SP)动物模型免疫功能的调控机制。方法:将60只小鼠分为对照组、模型组和观察组,其中模型组和观察组制备流感病毒感染的SP模型,在滴入H1N1第二天观察组使用复方芩兰口服液灌胃。HE染色ELISA检测肺组织损伤和炎性因子[白细胞介素1β(interlenkin-1β,IL-1β)、白细胞介素6(interlenkin-6,IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)],流式细胞术检测T辅助细胞17(T helper cells 17,Th17)和调节T细胞(regulatory T cells,Treg),qPCR和Western blot检测淋巴细胞中NFκB通路水平。结果:模型组及观察组肺指数、IL-1β、IL-6、TNF-α水平显著高于对照组(P<0.05),且观察组以上指标水平显著低于模型组(P<0.05)。模型组的Th17和Th17/Treg显著高于对照组而Treg显著低于对照组(P<0.05);观察组的Th17和Th17/Treg显著低于模型组而Treg显著高于模型组(P<0.05)。与对照组比较,模型组及观察组的RORγt mRNA,NF-κB、RelB mRNA及蛋白水平均高于对照组(P<0.05),FOXP3 mRNA水平低于对照组(P<0.05);且观察组的RORγt、NF-κB、RelB mRNA及蛋白水平显著低于模型组(P<0.05),FOXP3 mRNA水平高于模型组(P<0.05)。结论:复方芩兰口服液可以通过抑制NF-κB通路诱导Treg而抑制Th17,从而调节免疫炎性反应,缓解由流感病毒引起的SP。Objective:To explore the mechanism of compound Qinlan oral liquid regulating the immune function of animal models of severe pneumonia(SP)caused by influenza virus infection via NFκB pathway.Methods:Sixty mice were divided into the control group,the model group and the observation group,among them,SP models in the model group and the observation group were induced by influenza virus infection,the observation group were drenched with compound Qinlan oral liquid on the second day of H1N1 infusion.HE staining and ELISA were used to detect lung tissue injury and inflammatory factors(IL-1β,IL-6,TNFα),flow cytometry was adopted to detect Th17 and Treg,qPCR and Western blot to measure the levels of NF-κB pathway in lymphocyte.Results:Lung index,the levels of IL-1β,IL-6 and TNF-αof the model group and the observation group were notably higher than these of the control group(P<0.05),lung index,the levels of IL-1β,IL-6 and TNFαof the observation group were lower than these of the model group notably(P<0.05).Th17 and Th17/Treg of the model group were notably higher than these of the control group while Treg lower than that of the control group remarkably(P<0.05);Th17 and Th17/Treg of the observation group were lower than these of the model group while Treg higher than that of the model group(P<0.05).Compared with the control group,the levels of RORγt mRNA,NF-κB,RelB mRNA and protein of the model group and the observation group were higher than these of the control group(P<0.05),the levels of FOXP3 mRNA lower than these of the control group(P<0.05);and the levels of RORγt,NF-κB,RelB mRNA protein of the observation group were lower than these of the model group(P<0.05),the levels of FOXP3 mRNA higher than these of the model group(P<0.05).Conclusion:Compound Qinlan oral liquid could induce Treg through NFκB pathway to inhibit Th17,therefore to regulate immune inflammatory response and relieve SP induced by influenza virus.

关 键 词：流感病毒 重症肺炎 复方芩兰口服 NF-кB通路 调节T细胞 动物实验 

分 类 号：R563.1[医药卫生—呼吸系统]