大黄的炮制对生大黄、熟大黄5种游离蒽醌含量的影响测定  

Processing of Dahuang and Determination of Five Free Anthraquinones in Crude and Processed Dahuang

作　　者：张如 魏江存 马家宝 杨正腾 王成龙 ZHANG Ru;WEI Jiangcun;MA Jiabao;YANG Zhengteng;WANG Chenglong(Maternity and Child Health Care Hospital of Guangxi Zhuang Autonomous Region,Nanning 530003,China;Guangxi International Zhuang Medicine Hospital;The First Affiliated Hospital of Guangxi University of Chinese Medicine)

机构地区：[1]广西壮族自治区妇幼保健院,广西南宁530003 [2]广西国际壮医医院 [3]广西中医药大学第一附属医院

出　　处：《西部中医药》2021年第12期25-31,共7页Western Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81360524);2019年度广西高校中青年教师科研基础能力提升项目(2019KY0341);广西中医药大学青年基金项目(2019QN036);广西自然科学基金(2014GXNSFAA 118208);国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室[国学基金中医药发2009(21号)]。

摘　　要：目的:采用高效液相色谱法(high performance liquid chromatography,HPLC)测定生、熟大黄中的5种游离蒽醌的含量及差异,探讨中药炮制前后物质基础的变化规律。方法:使用色谱柱为Thermo BDS HYPERSII C_(18)柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸(85∶15)水溶液为流动相,检测波长为256 nm,流速1.0 mL/min,柱温30℃,进样量10μL。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.0207~0.414(R^(2)=0.9999)、0.1721~3.442(R^(2)=0.9996)、0.0203~0.406(R^(2)=0.9999)、0.148~2.96(R^(2)=0.9998)、0.164~3.28(R^(2)=0.9993)的线性范围内呈良好线性关系;生大黄经炮制后,芦荟大黄素含量下降约8.26%,大黄酸下降约9.21%;而大黄素含量增加约16.44%,大黄酚增加约16.12%,大黄素甲醚增加约20.01%,说明炮制工艺对大黄各成分影响不同。结论:大黄炮制后,其5种游离蒽醌的含量受到不同程度的影响,该方法可作为大黄及其不同炮制品的质量控制方法。Objective:To determine the contents and differences of five free anthraquinones in crude and processed Dahuang(Chinese rhubarb)using HPLC,and discuss the rules of material basis before and after the processing.Methods:Thermo BDS HYPERSII C_(18) Column(4.6mm×250mm,5μm)was used as the chroma tographic column,methanol-0.1%phosphoric acid(85:15)aqueous solution as the mobile phase,detection wavelength was 256nm,flow rate 1.0mL/min,column temperature 30℃,sample size 10μL.Results:Aloe emodin,rhein,emodin,chrysophanol,emodin methyl ether showed better linear relationship in the range between 0.0207 and 0.414(R^(2)=0.9999),0.1721 and 3.442(R^(2)=0.9996),0.0203 and 0.406(R^(2)=0.9999),0.148 and 2.96(R^(2)=0.9998),0.164 and 3.28(R^(2)=0.9993)respectively;after processing,the contents of aloe emodin decreased by about 8.26%,the contents of rhein decreased by about 9.21%;while the contents of emodin increased by around 16.44%,the contents of chrysophanol by about 16.12%and emodin methyl ether by about 20.01%,which demo-nstrated that the processing showed different influences on all the ingredients of Dahuang.Conclusion:After processing,the contents of five free anthraquinones are affected to varying degrees,and the method could be used for quality control of Dahuang and its processed products.

关 键 词：大黄 游离蒽醌 炮制 高效液相色谱法 

分 类 号：R283[医药卫生—中药学]