栀子提取物抑制甲型流感病毒致小鼠病毒性肺炎的抗炎机制研究  

Anti-inflammatory Mechanism of Gardenia Jasminoides Ellis Extract on Inhibition of Viral Pneumonia Induced by Influenza A Virus

作　　者：王雅欣 包蕾[1] 赵荣华[1] 耿子涵 鲍岩岩[1] 孙静[1] 时宇静[1] 周利润 陈梦苹 徐英莉 庞博 郭姗姗[1] 崔晓兰[1] WANG Yaxin;BAO Lei;ZHAO Ronghua;GENG Zihan;BAO Yanyan;SUN Jing;SHI Yujing;ZHOU Lirun;CHEN Mengping;XU Yingli;PANG Bo;GUO Shanshan;CUI Xiaolan(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药物警戒》2021年第12期1106-1110,1116,共6页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81773977)。

摘　　要：目的探索栀子提取物抑制甲型H1N1流感病毒(FM1株)致小鼠病毒性肺炎的抗炎机制。方法在体外实验中研究不同浓度的栀子提取物对脂多糖(LPS)诱导小鼠巨噬细胞RAW 267.4分泌一氧化氮(NO)的影响,用2种浓度的LPS分别刺激RAW 267.4细胞24 h,再分别给予相应浓度的栀子提取物,24 h后检测细胞上清液中NO的含量。在体内实验中:除正常组外,其余各组均用甲型H1N1流感病毒(FM1株)感染ICR小鼠肺炎模型,连续给药4 d后眼球取血离心收集血清做流式检测血清炎症因子,解剖后取肺组织做病理切片,观察肺部病变情况。结果在体外实验中,栀子提取物5、2.5、1.25 mg/mL 3个剂量组均可有效降低NO的含量,且与LPS对照组比较具有显著性差异(P<0.01)。在体内实验中,与模型组相比,栀子提取物高、中、低3个剂量组均可使炎症因子白介素-6(IL-6)、肿瘤坏死因子α(TNF-α)及单核细胞趋化蛋白-1(MCP-1)的含量有不同程度的降低,小鼠肺组织病理学检查表明,与模型组相比,栀子提取物高中剂量组均可明显改善肺部病变情况。结论栀子提取物不仅在体外实验中具有良好的抑炎作用,也可有效抑制甲型H1N1流感病毒(FM1株)所致的小鼠病毒性肺炎。Objective To explore the anti-inflammatory mechanism of Fructus Gardenia extract by which viral pneumonia caused by influenza A(H1 N1)virus(FM1)is inhibited in mice.Methods In vitro experiments:the effect of Fructus Gardenia extract of different concentrations on the secretion of NO was studied with mouse macrophage RAW 267.4 induced by lipopolysaccharide(LPS).The RAW 267.4 cells were stimulated by LPS of two concentrations for 24 hours and then by Fructus Gardeniae extract of corresponding concentrations.The content of NO in the cell supernatant was detected after 24 hours.In vivo experiments:Except for the normal group,the ICR mouse pneumonia model infected with influenza A(H1N1)virus(FM1)was used in all the other groups.After four days of continuous administration,blood was collected from the eyeball by centrifugation for flow cytometry detection of serum inflammatory factors.After dissection,lung tissue was taken for pathological section and lung lesions were observed.Results In vitro experiments showed that the 5,2.5 and 1.25 mg/mL dosage groups of Fructus Gardeniae extract could effectively reduce NO content,and there was significant difference compared with the LPS control group(P<0.01).In vivo experiments indicated that the high,medium and low dose groups of Fructus Gardeniae extract could reduce the contents of inflammatory factors interleukin-6(IL-6),tumor necrosis factorα(TNF-α)and monocyte chemoattractant protein-1(MCP-1)to different extents compared with the model group.The pathological examination of mouse lung tissue showed that compared with the model group,lung lesions were significantly improved in high and medium dose groups of Fructus Gardeniae extract.Conclusion Fructus Gardenia extract not only has a good anti-inflammatory effect in vitro,but also effectively inhibits mouse viral pneumonia caused by influenza A(H1N1)virus(FM1).

关 键 词：栀子提取物 甲型流感病毒 脂多糖 炎症因子 小鼠 

分 类 号：R961[医药卫生—药理学]