体外培育牛黄对人冠状病毒肺炎湿毒疫模型的机制研究  

The Mechanism of Cultured Bezoar against the Epidemic Model of Human Coronavirus Pneumonia

作　　者：赵荣华[1] 郭姗姗[1] 孙静[1] 时宇静[1] 包蕾[1] 耿子涵 鲍岩岩[1] 周冠儒 高英杰[1] 崔晓兰[1] 王道涵[3] 无 ZHAO Ronghua;GUO Shanshan;SUN Jing;SHI Yujing;BAO Lei;GENG Zihan;BAO Yanyan;Zhou Guanru;GAO Yingjie;CUI Xiaolan;WANG Daohan;无(Biosecurity Laboratory,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;Wuhan Jianmin Dapeng Pharmaceutical Co.,Ltd Wuhan 430000,China;Dongfang Hospital,Beijing University of Chinese Medicine,Beijing,100078,China)

机构地区：[1]中国中医科学院中药研究所生物安全实验室,北京100700 [2]武汉健民大鹏药业有限公司,武汉430000 [3]北京中医药大学东方医院儿科,北京100078

出　　处：《中国药物警戒》2021年第12期1117-1122,共6页Chinese Journal of Pharmacovigilance

基　　金：国家自然科学基金资助项目(81773977);国家自然科学基金培育专项(ZXKT20025)。

摘　　要：目的观察体外培育牛黄对人冠状病毒肺炎湿热疫毒袭肺证小鼠模型的治疗作用。方法通过体外培育牛黄(0.128、0.064、0.032 g/kg)对小鼠肺指数和肺指数抑制率、肺组织病毒载量、肺组织炎性因子白细胞介素(IL)-1β,白细胞分化抗原(cluster of differentiation,CD14),氧化应激因子丙二醛(MDA)及超氧化物歧化酶(SOD)含量、下丘脑环磷酸腺苷(cAMP)及前列腺素(prostaglandin,PG)E2含量、外周血CD4^(+)T淋巴细胞,CD8^(+)T淋巴细胞及B淋巴细胞百分比的影响评价药物对模型的治疗作用。结果人冠状病毒肺炎湿热疫毒袭肺证小鼠病证结合模型小鼠体重减轻,摄食及饮水量下降明显,扎堆不动,反应迟缓,皮毛干枯不泽,大便粘腻,小便量少色黄,体外培育牛黄可明显改善模型小鼠上述状态。体外培育牛黄可降低模型小鼠肺指数;明显降低模型小鼠肺组织病毒核酸表达量;显著降低模型小鼠肺组织炎性因子CD14、MDA含量,显著升高SOD含量;显著降低模型小鼠c AMP含量,对PGE2含量无显著变化;显著升高模型小鼠CD4^(+)T细胞、CD8^(+)T细胞的百分比。结论体外培育牛黄对人冠状病毒肺炎湿热疫毒袭肺证小鼠病证结合模型具有显著的治疗作用,可通过降低模型小鼠肺指数、调节下丘脑CAMP及PGE2的含量、调节免疫机能和抑制炎性因子释放等作用环节进行治疗,为临床用药提供实验室依据。Objective To observe the therapeutic effect of bezoar cultured in vitro on a mouse model of human coronavirus pneumonia caused by the lung-attacking damp-heat virus.Methods The therapeutic effect of bezoar cultured in vitro(0.128,0.064,0.032 g/kg)against a model was evaluated by comparing the effects of the drug on the lung index,inhibition rate of the lung index,viral load of lung tissue,contents of inflammatory factors IL-1β,CD14,MDA and SOD in lung tissue,contents of cAMP and PGE-2 in the hypothalamus,and on percentages of CD4^(+)T lymphocytes,CD8^(+)T lymphocytes and B lymphocytes in peripheral blood.Results The weight of the model mice was reduced,food and water intakes decreased significantly,the reaction of the mice became slow,their skin went dry and less lustrous,the stool was sticky,the amount of urine became smaller and looked yellow.Bezoar cultured in vitro reduced the lung index of model mice,significantly inhibited the expression of viral nucleic acid in lung tissue,significantly reduced the content of inflammatory factors CD14 and MDA in lung tissue,but significantly increased the content of SOD,significantly reduced the content of c AMP in the hypothalamus of model mice,hardly changed the content of PGE2,but significantly increased the percentage of CD4^(+)T cells and CD8^(+)T cells in model mice.Conclusion Bezoar cultured in vitro has significant therapeutic effect against the mouse model of the syndrome of human coronavirus pneumonia with lung-attacking damp-heat virus by reducing the lung index,regulating the contents of cAMP and PGE2 in the hypothalamus,regulating the immune function and inhibiting the release of inflammatory factors.

关 键 词：冠状病毒肺炎 体外培育牛黄 中医病机 湿热疫毒袭肺 免疫机能 炎性因子 

分 类 号：R994.11[医药卫生—毒理学]