单磷酸脂质A佐剂对无细胞百日咳疫苗的免疫保护效果研究  

Immune protective effect of monophosphoryl lipid A adjuvant on acellular pertussis vaccine

作　　者：王虓宇 江文文 胡文著 杭赛虎 赵宏 陈溢娟 赵云蓉 柏会文 赵宸 梁疆莉 WANG Xiao-yu;JIANG Wen-wen;HU Wen-zhu;HANG Sai-hu;ZHAO Hong;CHEN Yi-juan;ZHAO Yun-rong;BAI Hui-wen;ZHAO Chen;LIANG Jiang-li(Yunnan Key Laboratory of Vaccine Research&Development on Severe Infections Diseases,Institute of Medical Biology,Chinese Academy of Medical Science&Peking Union Medical College,Kunming 650118,China)

机构地区：[1]中国医学科学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,昆明650118

出　　处：《中国医药生物技术》2022年第1期12-18,共7页Chinese Medicinal Biotechnology

基　　金：云南省创新疫苗技术与产业转化研发平台(202002AA 100009);昆明市科学技术局科技创新中心示范建设计划重点项目(2019-1-N-25318000003332)。

摘　　要：目的探讨单磷酸脂质A(MPLA)佐剂对无细胞百日咳疫苗(aP)的免疫保护效果影响。方法 aP中添加MPLA佐剂,在Balb/c小鼠上进行免疫以及感染保护实验。通过检测小鼠百日咳特异性IgG抗体及其分型抗体IgG1和IgG2a水平、感染后白细胞变化以及气管和肺组织细菌定植来进行免疫效果的评估。结果 aP+MPLA免疫的小鼠在第一次和第二次免疫后所产生的IgG抗体水平均高于aP组,且两次基础免疫后显著促进了Th1细胞免疫。但百日咳气雾感染保护实验结果显示无论是否添加MPLA,疫苗在小鼠上都具有明显的保护效果。但添加MPLA佐剂,对小鼠感染后白细胞变化以及气管和肺组织细菌定植并未有显著改善。结论 MPLA佐剂对aP的免疫保护效果并未有明显改善。Objective We aim to investigate the effect of monophosphoryl lipid A(MPLA) adjuvant on the immune protection of acellular pertussis vaccine(aP).Methods MPLA was added to aP, and the immunity and infection protection were evaluated on Balb/c mice. The immune effect was evaluated by detecting the titers of B.pertussis-specific IgG antibodies and their typing antibodies IgG1 and IgG2 a, dynamics of white blood cells and bacterial colonization in trachea and lung tissues after B.pertussis infection.Results The a P with MPLA-immunized mice group produced higher levels of IgG antibodies after the first and second immunizations than the aP alone group. After two basic immunization procedures, MPLA significantly promoted Th1 cell immunity.However, the results of pertussis infection showed that the vaccines had a significant protective effect in mice regardless of the addition of MPLA. However, the addition of MPLA did not significantly improve the changes of white blood cells and bacterial colonization in trachea and lung tissues after infection.Conclusion The immune protection effect of MPLA adjuvant on aP is not significantly improved.

关 键 词：百日咳鲍特氏杆菌 无细胞百日咳疫苗 单磷酸脂质A 佐剂 

分 类 号：R392-3[医药卫生—免疫学]