Optimization of preparation process of cationic liposome nanoparticles containing Survivin-siRNA and osthol  

作　　者：Qi-Chao Sun Hao-Tian Bai Ya-Lan Li Jian-Nan Meng Yu-Wei Guo Rui Wang 

机构地区：[1]The First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,China [2]Heilongjiang University of Traditional Chinese Medicine,Harbin 150040,China

出　　处：《Journal of Hainan Medical University》2022年第1期7-13,共7页海南医学院学报（英文版）

基　　金：Harbin Applied Technology Research and Development Project(No.2016RAQXJ197)。

摘　　要：Objective:To prepare cationic liposome nanoparticles loaded with survivin-siRNA and Cnidium monnieri based on the ability of liposomes to contain both water-soluble and lipid soluble components.Methods:The preparation technology of Osthol cationic liposomes was optimized by orthogonal test with membrane material ratio,drug lipid ratio,ultrasonic time and steaming temperature as factors.The volume ratio of HA-siRNA to protamine and the ratio of HA-siRNA protamine complex to liposome were investigated by control variable method with potential and particle size as indexes.The particle size and zeta potential were measured by potentiometric particle size analyzer,and the shape was observed by transmission electron microscope;The absorbance of different concentrations of FAM-Survivin-siRNA standard solution was measured by microplate analyzer,and the entrapment efficiency of cationic liposomes loaded with FAM-Survivin-siRNA and osthole was calculated.Results:The optimum preparation conditions of osthole loaded cationic liposomes were as follows:the ratio of membrane to material was 3:1,the ratio of drug to lipid was 1:5,the steaming temperature was 30℃,the ultrasonic time was 70 min,and the encapsulation efficiency was 78.34%.The optimum preparation conditions of osthole loaded cationic liposomes loaded FAM-Survivin-siRNA were as follows:the volume ratio of Survivin-siRNA to protamine was 1:1,Protamine complex 25μg.Add 50μL cationic liposomes.The particle size is 132.3±0.2nm,zeta potential is 43.15±0.05mv,and its shape is irregular round;According to the standard curve,the entrapment efficiency of cationic liposome nanoparticles co loaded with Survivin-siRNA and osthole was 81.34±0.041%.Conclusion:The prepared cationic liposome nanoparticles loaded with Survivin-siRNA and osthole have good encapsulation efficiency,particle size and zeta potential.

关 键 词：SIRNA Cationic liposomes Film dispersion method Preparation technology 

分 类 号：O64[理学—物理化学]