碳水化合物结合模块-嗜热子囊菌角质酶融合蛋白在PET降解中的应用  

The application of carbohydrate binding module-Thermobifida fusca cutinase fusion protein in polyethylene terephthalate degradation

作　　者：张颖[1,2,3] 刘展志 李光耀 付雪妮 张钰成 王志远 田亚平 吴敬[1,2,3] ZHANG Ying;LIU Zhanzhi;LI Guangyao;FU Xueni;ZHANG Yucheng;WANG Zhiyuan;TIAN Yaping;WU Jing(State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu,China;Key Laboratory of Industrial Biotechnology,Ministry of Education,School of Bioengineering,Jiangnan University,Wuxi 214122,Jiangsu,China;Joint Laboratory for International Cooperation in Food Safety by the Ministry of Education,Jiangnan University,Wuxi 214122,Jiangsu,China)

机构地区：[1]江南大学食品科学与技术国家重点实验室,江苏无锡214122 [2]江南大学生物工程学院工业生物技术教育部重点实验室,江苏无锡214122 [3]江南大学教育部食品安全国际合作联合实验室,江苏无锡214122

出　　处：《生物工程学报》2022年第1期217-225,共9页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(2019YFA0706900);江苏省科技厅政策引导类计划(国际科技合作/港澳台科技合作)—“一带一路”创新合作项目(BZ2020010)。

摘　　要：随着全球经济的发展,聚对苯二甲酸乙二醇酯(PET)塑料的生产量大幅提高,其废弃总量也逐年递增。废弃PET处理方法主要包括填埋、焚烧及生物降解等。填埋和焚烧均会造成二次污染,而生物降解因其环境友好特性逐渐成为研究热点。相关研究表明,碳水化合物结合模块(carbohydrate binding module,CBM)可以有效增强PET降解酶对PET的吸附,从而增加降解速率。通过大引物PCR (megaprimer PCR of whole plasmids,MEGAWHOP)技术,将炭疽杆菌(Bacillus anthraci)来源的CBM与嗜热子囊菌(Thermobifida fusca)角质酶(Tfuc)构建融合蛋白Ba CBM2-Tfuc并表征其PET降解性能。在60℃条件下,Ba CBM2-Tfuc降解PET膜的效率是Tfuc的2.8倍。本研究可为构建高效降解PET的融合蛋白提供技术支持。With the development of global economy, the dramatically increased production of polyethylene terephthalate(PET) plastics has led to a remarkably increased amount of plastic waste.PET waste can be treated by landfill, incineration, or biodegradation. While landfilling and incineration may cause secondary pollution, biodegradation has since received increased attentions due to its environmental friendliness. Recent studies have indicated that the carbohydrate binding module(CBM)can effectively enhance the binding of PET degrading enzymes to PET, and consequently increasing PET degradation rate. Here we constructed a fusion protein Ba CBM2-Tfuc containing the Ba CBM2 from Bacillus anthraci and the cutinase Tfuc from Thermobifida fusca, by megaprimer PCR of whole plasmids(MEGAWHOP). Notabaly, the PET film degradation efficiency(at 60 ℃) of Ba CBM2-Tfuc was 2.8 times that of Tfuc. This study may provide technical support for constructing fusion proteins capable of efficiently degrading PET.

关 键 词：聚对苯二甲酸乙二醇酯 生物降解 角质酶 碳水化合物结合模块 融合蛋白 

分 类 号：X705[环境科学与工程—环境工程]