萝卜TALE转录因子家族的鉴定与分析  

Identification and analysis of the TALE transcription factor family in radish

作　　者：赵伟 李锡香[1] 王海平[1] 贾会霞 宋江萍[1] 阳文龙 张晓辉[1] ZHAO Wei;LI Xixiang;WANG Haiping;JIA Huixia;SONG Jiangping;YANG Wenlong;ZHANG Xiaohui(Beijing Research Station of Vegetable Crop Gene Resource and Germplasm Enhancement,Ministry of Agriculture and Rural Affairs of People’s Republic of China,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture and Rural Affairs of People’s Republic of China,Institute of Vegetables and Flowers,Chinese Academy of Agriculture Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业农村部园艺作物生物学与种质创制综合重点实验室,农业农村部蔬菜作物基因资源与种质创制北京科学观测实验站,北京100081

出　　处：《生物工程学报》2022年第1期343-358,共16页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(31772301);国家重点研发计划(2016YFD0100204-07);基本科研业务费(IVF-BRF2020001)。

摘　　要：TALE (three-amino acid loop extension)转录因子在植物生长发育及细胞分化过程中起重要作用。在多种植物中均已鉴定出TALE转录因子的家族成员,但是萝卜TALE转录因子家族的研究鲜有报道。文中通过生物信息学手段在象牙白萝卜全基因组中鉴定出了分布于9条染色体上的33个TALE家族基因。研究结果显示,该家族中除与拟南芥KNATM同源的基因Rsa10037940外,其余基因均含有编码HOX保守结构域的序列。这些基因含有4–6个外显子。萝卜的33个TALE基因与拟南芥中的17个同源基因存在共线性关系。33个TALE基因启动子区的顺式元件中包含大量逆境响应元件。表达特性分析显示,该基因家族BELL亚家族内有4个基因在根内表达量较高,KNOX亚家族内有2个基因在薹和愈伤中表达量较高。该家族不同亚型成员之间的蛋白3D结构高度相似。编码蛋白均为弱酸、有亲水性。萝卜TALE基因家族在进化上较为保守,分化上与拟南芥存在一致性,与水稻差异较大。本研究为开展萝卜中TALE转录因子的生物学功能研究提供了重要参考。Three-amino acid loop extension(TALE) transcription factors play important roles in plant growth and cell differentiation. There are plenty of studies on TALE transcription factors in several model plants,but not in radish(Raphanus sativas). A genome-wide bioinformatics analysis identified 33 TALE family genes in the Xiang-Ya-Bai(XYB) radish, These genes,are distributed on nine chromosomes and all contain 4-6 exons. The 33 TALE genes in radish showed a co-linearity relationship with the 17 homologous genes in Arabidopsis thaliana. Moreover, a large number of stress response cis-elements were found in the promoter regions of these genes. Expression analysis showed that four genes in the BELL subfamily were highly expressed in roots, and two genes in the KNOX subfamily were highly expressed in shoots of bolting plants and callus. All radish TALE genes contain sequences encoding the conserved HOX domain,except for the gene RSA10037940, which is homologous to Arabidopsis KNATM. The deduced 3 D structures of the TALE proteins irrespective of subtypes are highly similar. All the encoded proteins were weakly acidic and hydrophilic. The radish TALE gene family is relatively evolutionarily conserved, which was consistent with results from Arabidopsis, but quite different from that of rice. This study provides important clues for studying the biological functions of TALE transcription factors in radish.

关 键 词：萝卜 TALE基因家族 基因结构 进化分析 组织表达 

分 类 号：S631.1[农业科学—蔬菜学]