银杏叶提取物通过抑制炎症和调节肠杆菌群落减轻急性胰腺炎大鼠的肠黏膜屏障损伤的机制  

Ginkgo biloba extract reduces intestinal mucosal barrier damage in rats with pancreatitis by inhibiting inflammation and regulating enterobacteria community

作　　者：高吟 张立红[1] 胡锡池[2] 胡仁静[2] 黄新祥[3] GAO Yin;ZHANG Li-hong;HU Xi-chi(Department of Laboratory Medicine,Wuxi Hospital of Traditional Chinese Medicine,Wuxi Jiangsu 214000,China;Department of Laboratory Medicine,Wuxi Second People's Hospital,Wuxi Jiangsu 214000,China)

机构地区：[1]无锡市中医医院检验科,江苏无锡214000 [2]无锡市第二人民医院检验科,江苏无锡214000 [3]江苏大学医学院,江苏镇江212000

出　　处：《临床和实验医学杂志》2022年第1期18-22,共5页Journal of Clinical and Experimental Medicine

基　　金：江苏省自然科学基金项目(编号:BK20191429);无锡市卫健委青年项目(编号:Q201840)。

摘　　要：目的探究银杏叶提取物(GBE)通过抑制炎症和调节肠杆菌群落减轻急性胰腺炎(AP)大鼠的肠黏膜屏障损伤的机制。方法 45只雄性Sprague Dawley大鼠购自医学院研究中心,用牛磺胆酸钠诱导大鼠AP模型。将实验大鼠分为对照组、AP组和GBE治疗组,每组15只。对照组大鼠给予假手术处理,并给予0.9%氯化钠溶液作为实验对照,AP组使用牛磺胆酸钠诱导大鼠AP,GBE治疗组诱导AP前给予GBE进行治疗,持续3 d。使用QIAamp DNA粪便试剂盒从大鼠粪便样本中提取细菌基因组DNA,并根据提取物的DNA纯度分析大鼠肠杆菌群。苏木精和伊红染色评估大鼠胰腺和小肠组织损伤。通过酶联免疫吸附试验测定试剂盒检测3组大鼠血浆炎症因子肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-β、IL-6和IL-12的水平。通过血浆FITC-葡聚糖水平评估大鼠肠通透性。通过蛋白质印迹法分析肠屏障相关蛋白的表达。结果 AP组大肠杆菌、克雷伯杆菌、阴沟肠杆菌和科贝肠杆菌的细菌数量较对照组升高,GBE治疗组大肠杆菌、克雷伯杆菌、阴沟肠杆菌和科贝肠杆菌数量较AP组减少,差异均有统计学意义(P <0.05)。AP组胰腺和小肠组织损伤评分较对照组升高,GBE治疗组胰腺和小肠组织损伤评分较AP组降低,差异均有统计学意义(P <0.05)。AP组TNF-α、IL-β、IL-6和IL-12水平较对照组降低,GBE治疗组TNF-α、IL-β、IL-6和IL-12水平较AP组降低,差异均有统计学意义(P <0.05)。AP组淀粉酶活性、二胺氧化酶和脂多糖浓度较对照组升高,GBE治疗组淀粉酶活性、二胺氧化酶和脂多糖浓度较对AP组降低,差异均有统计学意义(P <0.05)。AP组FITC-葡聚糖较对照组升高,GBE治疗组FITC-葡聚糖较AP组降低,差异均有统计学意义(P <0.05)。AP组ZO-1、occludin和claudin-1蛋白表达较对照组降低,MMP-9蛋白表达较对照组升高,GBE治疗组ZO-1、occludin和claudin-1蛋白表达较AP组升高,MMP-9蛋白表达�Objective To explore the mechanism by which Ginkgo biloba extract reduces the intestinal mucosal barrier damage in rats with acute pancreatitis( AP) by inhibiting inflammation and regulating the enterobacteria community. Methods Forty-five male Sprague Dawley rats were purchased from the Research Center of the Medical College,and sodium taurocholate was used to induce an AP model in large rats. The experimental rats were divided into control group,AP group and GBE treatment group,each 15 rats. The rats in the control group were given a sham operation and 0. 9% sodium chloride solution as an experimental control. The AP group was treated with sodium taurocholate to induce AP,and the GBE treatment group was treated with GBE before AP induction for 3 days. The QIAamp DNA stool kit was used to extract bacterial genomic DNA from rat stool samples,and the rat enterobacteria population was analyzed based on the DNA purity of the extract. Hematoxylin and eosin staining was used to assess pancreatic and small intestinal tissue damage in rats. The plasma levels of TNF-α,IL-β,IL-6 and IL-12 were detected by enzyme-linked immunosorbent assay kit. The intestinal permeability of rats was evaluated by plasma FITC-dextran level. The expression of intestinal barrier-related proteins was analyzed by Western blotting. Results The number of Escherichia coli,Klebsiella,Enterobacter cloacae,and Enterobacter Klebsiella in the AP group was higher than that in the control group,the number of Enterobacter shellfish was lower than that in the AP group,the differences were statistically significant( P < 0. 05). The pancreatic and small intestine tissue damage scores in the AP group were higher than those in the control group,and the pancreatic and small intestine tissue damage scores in the GBE treatment group were lower than those in the AP group,the differences were statistically significant( P < 0. 05). The levels of TNF-α,IL-β,IL-6 and IL-12 in the AP group were lower than those in the control group,and the levels of inflammatory fa

关 键 词：大鼠 银杏叶提取物 肠杆菌群 急性胰腺炎 肠黏膜屏障 炎症 

分 类 号：R285.5[医药卫生—中药学]