松材线虫诱导棘孢木霉T203抗病相关基因的转录组研究  

Transcriptome Study on the Disease Resistance Related Genes Induced by Bursaphelenchus xylophilus in Trichoderma asperellum T203

作　　者：焦宁 尹大川[2] 宋瑞清[1] JIAO Ning;YIN Da-chuan;SONG Rui-qing(Forestry College,Northeast Forestry University,Harbin 150040,China;Forestry College,Shenyang Agricultural University,Shenyang 110161,China)

机构地区：[1]东北林业大学林学院,哈尔滨150040 [2]沈阳农业大学林学院,沈阳110161

出　　处：《沈阳农业大学学报》2021年第6期677-688,共12页Journal of Shenyang Agricultural University

基　　金：国家林业局“948”项目(2015-4-34)。

摘　　要：由松材线虫(Bursaphelenchus xylophilus)引起的松树萎蔫病(pine wilt disease,PWD)严重危害松属植物,给我国林业资源造成巨大损失。棘孢木霉(Trichoderma asperellum)对松材线虫有抑制作用。通过对松材线虫处理的棘孢木霉(Trichoderma asperellum)T203菌株转录组分析,为棘孢木霉的分子抗病机制提供理论依据,也为松材线虫的生物防治提供新的思路。以松材线虫悬浮液与无菌水处理的棘孢木霉T203菌丝为样本,利用RNA-Seq技术分析棘孢木霉T203的差异表达基因。结果表明:棘孢木霉T203测序数据共得到964个差异表达基因,其中,371个上调表达,583个下调表达。GO功能富集到氨基酸转运、蛋白质合成与代谢、细胞合成以及催化酶活性等相关功能的基因,KEGG代谢途径富集到抗生素生物合成、氨基酸代谢、谷胱甘肽代谢、过氧化物酶体等相关功能的基因。在MAPK信号途径中发现了编码信号识别传递与诱导抗病机制的G蛋白、过氧化氢酶等基因。在差异表达基因中筛选出了12个松材线虫诱导棘孢木霉T203的抗病基因,qRT-PCR验证表明这些基因与转录组数据表达模式一致。获取了高质量棘孢木霉T203的转录组测序结果,棘孢木霉主要通过抗病基因编码信号传导因子、抗氧化酶、水解酶、解毒酶、抗生素等活性代谢产物调控自身防卫反应。MAPK信号途径在棘孢木霉菌丝生长发育、信号识别传导以及诱导抗病机制等方面具有重要的意义。Pine wilt disease(PWD)caused by Bursaphelenchus xylophilus,seriously damages pine plants and causes huge loss of forestry resources in China.Trichoderma asperellum has an inhibitory effect on Bursaphelenchus xylophilus.To provide a theoretical basis for the molecular resistance mechanism of Trichoderma asperellum by analyzing the transcriptome of Trichoderma asperellum T203 and provide a new idea for the biological control.The differentially expressed genes of T.asperellum T203 treated with B.xylophilus suspension and sterile water were analyzed by RNA-Seq.The result show:a total of 964 differentially expressed genes were obtained from T.asperellum T203 sequencing data,of which 371 genes were up-regulated and 583 genes were down-regulated.Go function is enriched in genes related to amino acid transport,protein synthesis and metabolism,cell synthesis and catalytic enzyme activity,while KEGG pathway is enriched in genes related to antibiotic biosynthesis,amino acid metabolism,glutathione metabolism and peroxisome.The genes encoding G protein and catalase were found in MAPK signaling pathway.Among the differentially expressed genes,12 genes from Bursaphelenchus xylophilus induced disease resistance in Trichoderma asperellum T203 were screened.qRT-PCR showed that the expression patterns of these genes were consistent with the transcriptome data.High quality transcriptome sequencing results of T.asperellum T203 were obtained,T.asperellum mainly regulates its defense response by encoding signal transduction factors,antioxidant enzymes,hydrolases,detoxifying enzymes,antibiotics and other active metabolites.MAPK signaling pathway plays an important role in the development of T.asperellum filaments,signal recognition and transduction,and the mechanism of inducing disease resistance.

关 键 词：松材线虫 棘孢木霉T203 转录组测序 抗病基因 

分 类 号：S763.11[农业科学—森林保护学]