硫化钠对大鼠经口给药的毒性和致畸性检测  

Toxicological test of orally administered sodium sulfide in rats

作　　者：夏祺悦 岳茜岚 豆捷雄 庄思琪 刘科亮[1] XIA Qiyue;YUE Qianlan;DOU Jiexiong;ZHUANG Siqi;LIU Keliang(Sichuan Center for Disease Control and Prevention,Chengdu 610041,Sichuan,China)

机构地区：[1]四川省疾病预防控制中心,四川成都610041

出　　处：《癌变．畸变．突变》2022年第1期62-66,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:研究硫化钠对大鼠经口给药的急性毒性、长期毒性和致畸性,为其进一步的毒理学研究及应用提供参考。方法:选用SPF级SD大鼠,雌雄各半,采用霍恩法设置215、464、1000、2150 mg/kg剂量系列经口给药检测大鼠急性毒性;常规重复给药毒性试验剂量分别为30、60、120 mg/kg,另外设蒸馏水对照组,检测亚慢性毒性;在妊娠中期采用剂量分别为30、60和120 mg/kg连续给药10 d的传统致畸试验检测其致畸毒性。结果:硫化钠对大鼠急性经口LD_(50)为雌性794(584~1080)mg/kg,雄性926(636~1350)mg/kg;大鼠重复给药毒性试验未出现中毒症状及死亡,120 mg/kg高剂量组大鼠的消化道出现病理学改变。与对照组比较,致畸试验120 mg/kg剂量组发现死胎百分率(1.75%)和外观畸形窝发生率(5.88%)均显著增加(P<0.05)。结论:在本试验条件下,硫化钠经口给药急性毒性LD_(50)分别为雌性794 mg/kg、雄性926 mg/kg;重复给药毒性试验中(≤60 mg/kg时)未观察到有害作用,致畸试验出现可疑阳性。OBJECTIVE:Studies on acute toxicity,repeated toxicity and teratogenicity of sodium sulfide after oral administration in rats.METHODS:SPF SD rats were selected,half male and half female.Horne's test was used to evaluate acute toxicity with dosing series of 215,464,1000,2150 mg/kg sodium sulfide.To study sub-chromic oral toxicity,rats were administered with the doses of 30,60 and 120 mg/kg.Teratogenicities were measured by conventional teratogenicity test with continuous administration for 10 days with 30,60 and 120 mg/kg in the second trimester.RESULTS:In the oral administration of sodium sulfide,LD_(50) value and 95%confidence intervals were 794(584-1080)mg/kg for female and 926(636-1350)mg/kg for male.In the teratogenicity test,the rate of stillbirth(1.75%)and the rate of deformity(5.88%)were significantly increased in the high-dose group of 120 mg/kg(P<0.05),compared with the control group.CONCLUSION:The LD_(50) of female and male SD rats were 794 mg/kg and 926 mg/kg respectively.In the repeated toxicity test,pathological changes in digestive tract existed in the high-dose group of 120 mg/kg.The no observed adverse effect level(NOAEL)in repeated administration toxicity tests was 60 mg/kg.Suspicious positive results occurred in the teratogenic tests.

关 键 词：硫化钠 急性毒性 重复给药毒性试验 致畸试验 

分 类 号：R994.6[医药卫生—毒理学]