长叶红砂RtVAMP2-2基因克隆及功能验证  

Cloning and functional validation of RtVAMP2-2 gene of Reaumuria trigyna

作　　者：张健 王彩霞 王迎春[1] 郑琳琳[1] ZHANG Jian;WANG Caixia;WANG Yingchun;ZHENG Linlin(College of Life Sciences,Inner Mongolia University,Hohhot 010000,Inner Mongolia,China)

机构地区：[1]内蒙古大学生命科学学院,内蒙古呼和浩特010000

出　　处：《草业科学》2021年第12期2363-2371,共9页Pratacultural Science

基　　金：国家自然科学基金(31860669);内蒙古自治区高等学校“青年科技英才支持计划”(NJYT-19-B04)。

摘　　要：长叶红砂(Reaumuria trigyna)是一种珍稀泌盐盐生植物,其特有的盐腺结构是长叶红砂适应盐渍荒漠环境的关键,膜泡运输参与该植物的盐腺分泌过程。本研究基于盐胁迫下长叶红砂的转录组数据,克隆获得膜泡运输相关基因RtVAMP2-2。生物信息学分析发现,RtVAMP2-2基因的开放阅读框为1074 bp,编码357个氨基酸;亚细胞定位和表达特性分析表明,该基因定位于细胞质膜,其表达受盐胁迫诱导;构建植物表达载体,将RtVAMP2-2基因转入拟南芥(Arabidopsis thaliana)中进行功能鉴定,结果显示,转基因拟南芥呈现盐敏感的表型,推测RtVAMP2-2基因可能在植物耐盐中发挥负调控作用。Reaumuria trigyna is a rare recretohalophyte.Its unique salt-gland structure plays a key role in its adaptation to saline desert environment,and vesicular trafficking is involved in the salt secretion process of the plant.In this study,RtVAMP2-2 gene involved in membrane vesicle trafficking was cloned based on the analysis of transcriptome data of R.trigyna under salt stress.The ORF of RtVAMP2-2 was 1074 bp and encoded 357 amino acids.RtVAMP2-2 was localized to the plasma membrane,and its expression was induced by saline stress.Then RtVAMP2-2 was transferred into Arabidopsis thaliana for functional validation.The results showed that the transgenic Arabidopsis thaliana showed a salt-sensitive phenotype,and was speculated that the RtVAMP2-2 may have a negative regulatory effect on plant salt tolerance.

关 键 词：转基因 盐腺 膜泡运输 盐胁迫 VAMP 泌盐盐生植物 长叶红砂 

分 类 号：S793.9[农业科学—林木遗传育种]