一年生黑麦草遗传多样性分析及新品系‘川饲1号’指纹图谱构建  

DNA fingerprinting of annual ryegrass strain of‘Chuansi No.1’using SSR markers

作　　者：程少波 宋阳 杨巽喆 马啸[2] 周永红[1,3] 张海琴 CHENG Shaobo;SONG Yang;YANG Xunzhe;MA Xiao;ZHOU Yonghong;ZHANG Haiqin(Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;College of Grassland Science and Technology,Sichuan Agricultural University,Chengdu 611130,Sichuan,China;State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China,Sichuan Agricultural University,Chengdu 611130,Sichuan,China)

机构地区：[1]四川农业大学小麦研究所,四川成都611130 [2]四川农业大学草业科技学院,四川成都611130 [3]西南作物基因资源发掘与利用国家重点实验室,四川成都611130

出　　处：《草业科学》2021年第12期2381-2389,共9页Pratacultural Science

基　　金：国家自然科学基金(31870309);四川省科技项目(2021NZZJ0010、21YYJSYJ0083)。

摘　　要：利用SSR分子标记构建23个一年生黑麦草(Lolium multiflorum)品种(系)的指纹图谱,以实现对一年生黑麦草新品系‘川饲1号’的准确及快速分子鉴定。12对SSR引物共扩增出104条谱带,多态性信息量(PIC)平均为0.613,变幅为0.392~0.822;香农指数和基因多样性指数变化范围分别为0.911~2.061和0.407~0.838,平均为1.325和0.650。聚类分析表明:23份材料聚为三大支,第一大支包括新品系‘川饲1号’及其5个亲本群体,其中‘川饲1号’与‘PI 611146’(母本,‘Billion’)和‘杰威’聚为一小支,‘PI 283610’、‘PI 283612’和‘PI 376875’(‘G.Manawa’)聚为另一小支;第二大支为‘赣饲3号’、‘盐城’等12个国审品种;第三大支为‘邦德’、‘特高’等5份国审品种。综上可知‘川饲1号’与亲本供体(特别是母本‘Billion’)亲缘关系较近,而与其他17个一年生黑麦草国审品种差异较大。研究发现引物02-12E在‘川饲1号’新品系上扩增出特有条带,可用于品种指纹图谱的快速构建和品种分子鉴定。DNA fingerprinting of 23 varieties of annual ryegrass(Lolium multiflorum)were constructed using the SSR markers in this study.A total of 104 bands were amplified using 12 SSR primer pairs.The polymorphism information content(PIC)ranged from 0.392 to 0.822,with an average of 0.613.The Shannon index and gene diversity index ranged from 0.911 to 2.061 and from 0.407 to 0.838,respectively,with averages of 1.325 and 0.650,respectively.Cluster analysis showed that all the materials were divided into three branches.The first branch included a new line of‘Chuansi No.1’and its parental donor,in which‘Chuansi No.1’was clustered with‘PI 611146’and‘Splendor’as a small branch,and with‘PI 283610’,‘PI 283612’,and‘PI 376875’(‘G.Manawa’)as another small branch.The second largest branch was comprised of 12 nationally approved varieties,including‘Gansi No.3’and‘Yancheng’.The third largest branch included five nationally approved varieties such as‘Abundant’and‘Tetragold’.These results suggested that high genetic variation existed between the new line of‘Chuansi No.1’and the cultivated varieties of L.multiflorum used in this study.Primer 02-12E amplified unique bands on‘Chuansi No.1’,which can be used for the rapid construction of fingerprints and molecular identification of this new line.

关 键 词：SSR标记 指纹图谱 一年生黑麦草 新品种(系) 遗传多样性 聚类分析 分子鉴定 

分 类 号：S544.9[农业科学—作物学]