自贡地区表观健康成人血清PGⅠ、PGⅡ和PGR参考区间的建立  

Establishment of reference intervals of serum PGⅠ,PGⅡ and PGR for apparently healthy subjects in Zigong

作　　者：余建洪[1] 彭庆远 殷明刚 YU Jianhong;PENG Qingyuan;YIN Minggang(Department of Clinical Laboratory,Zigong First People's Hospital,Zigong 643000,Sichuan,China)

机构地区：[1]自贡市第一人民医院检验科,四川自贡643000

出　　处：《检验医学》2022年第1期23-27,共5页Laboratory Medicine

基　　金：四川省卫生和计划生育委员会科研基金资助项目(17PJ526);自贡市重点科技计划项目(2017SF09)。

摘　　要：目的建立自贡地区表观健康成人血清胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)和胃蛋白酶原Ⅰ/胃蛋白酶原Ⅱ比值(PGR)的参考区间,为临床相关疾病诊治提供依据。方法选取自贡市第一人民医院表观健康体检者2880名,采用胶乳增强免疫比浊法检测PGⅠ、PGⅡ水平,并计算PGR,同时采用^(13)C尿素呼气试验筛查幽门螺杆菌(HP)的感染情况。采用百分位数法(P_(2.5)~P_(97.5))建立参考区间。采用受试者工作特征(ROC)曲线评价PGⅠ、PGⅡ判断HP阳性的效能。采用Kendall’s tau-b相关分析评价PGⅠ、PGⅡ与HP阳性的相关性。结果男性血清PGⅠ、PGⅡ水平均高于女性(P<0.05),PGR低于女性(P<0.05)。18~44岁组、45~59岁组、>59岁组血清PGⅠ和PGⅡ水平依次升高,各组间差异均有统计学意义(P<0.05);各年龄组PGR差异均无统计学意义(P>0.05)。血清PGⅠ、PGⅡ根据性别和年龄分别建立参考区间,PGR按性别建立参考区间。以13C尿素呼气试验阳性作为HP阳性的依据,ROC曲线分析结果显示,PGⅠ、PGⅡ和PGR判断HP阳性的曲线下面积分别为0.712、0.825和0.799。相关性分析结果显示,PGⅠ、PGⅡ与HP阳性均呈正相关(r值分别为0.286、0.440,P<0.01),PGR与HP阳性呈负相关(r=-0.403,P<0.01)。结论初步建立了自贡地区表观健康成人血清PGⅠ、PGⅡ及PGR的参考区间。Objective To establish the reference intervals of serum pepsinogenⅠ(PGⅠ),pepsinogenⅡ(PGⅡ)and the ratio of pepsinogenⅠ/pepsinogenⅡ(PGR)for apparently healthy subjects in Zigong,and to provide a reference for clinical diagnosis and treatment.Methods The levels of PGⅠand PGⅡwere determined by latex enhanced immunoturbidimetry,^(13)C urea breath test was used to screen Helicobacter pylori(HP)infection in 2880 apparently healthy subjects from Zigong First People’s Hospital,and PGR was calculated.The reference interval was established by percentile method(P_(2.5)-P_(97.5)).The efficacy of PGⅠand PGⅡin judging HP positivity was evaluated by receiver operating characteristic(ROC)curve.Kendall’s tau-b correlation analysis was used to evaluate the correlation between PGⅠ,PGⅡand HP positivity.Results The levels of serum PGⅠand PGⅡin males were higher than those in females(P<0.05),and PGR was lower(P<0.05).The levels of serum PGⅠand PGⅡin 18-44-year-old group,45-59-year-old group and>59-year-old group increased in turn(P<0.05).There was no statistical significance for PGR among the age groups(P>0.05).The reference intervals of serum PGⅠand PGⅡwere established according to sex and age,and the reference intervals of PGR were established according to sex.Taking the positive 13C urea breath test as the basis for HP positivity,the areas under curves for HP positivity judged by PGⅠ,PGⅡand PGR were 0.712,0.825 and 0.799,respectively.There were correlations between PGⅠ,PGⅡand HP positivity(r=0.286 and 0.440,respectively,P<0.01),while PGR was negatively correlated with HP positivity(r=-0.403,P<0.01).Conclusions The reference intervals of serum PGⅠ,PGⅡand PGR in apparently healthy subjects in Zigong have been established.

关 键 词：胃蛋白酶原Ⅰ 胃蛋白酶原Ⅱ 参考区间 

分 类 号：R446.1[医药卫生—诊断学]