胶体金免疫层析法快速检测CRE碳青霉烯酶效果评价  

Evaluation of colloidal gold immunochromatography assay for the rapid determination of carbapenemase in CRE isolates

作　　者：姚锂凤 郑巧平 杨蒙 罗清琼[1] 陈旭[1] 陈福祥[1] YAO Lifeng;ZHENG Qiaoping;YANG Meng;LUO Qingqiong;CHEN Xu;CHEN Fuxiang(Department of Clinical Laboratory,Shanghai Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院检验科,上海200011

出　　处：《检验医学》2022年第1期68-71,共4页Laboratory Medicine

基　　金：上海市青年科技英才扬帆计划(19YF1427500);上海市“医苑新星”青年医学人才培养计划。

摘　　要：目的评估胶体金免疫层析法(GICA)快速检测耐碳青霉烯类肠杆菌科细菌(CRE)碳青霉烯酶类别的临床应用价值。方法收集CRE非重复临床分离株73株,采用聚合酶链反应(PCR)鉴定碳青霉烯酶基因型,并采用GICA快速检测CRE的碳青霉烯酶。以PCR为参考方法,评估GICA快速检测CRE碳青霉烯酶的准确性。结果PCR检测结果显示,73株CRE中有51株(69.9%)bla_(KPC)基因阳性,19株(26.0%)bla_(NDM)基因阳性,2株(2.7%)bla_(IMP)基因阳性,1株(1.4%)bla_(VIM)基因阳性,未检测到bla_(OXA)基因阳性菌株。GICA对bla_(KPC)、bla_(NDM)、bla_(IMP)及bla_(VIM)阳性菌株酶型检测的准确性均为100%。GICA与PCR在检测CRE碳青霉烯酶类型中的一致性为100%。结论GICA具有简便、快速、准确度高等特点,在早期监测耐碳青霉烯类菌株和临床抗感染治疗中具有重要的应用价值。Objective To evaluate the clinical application role of colloidal gold immunochromatography assay(GICA)for the rapid determination of carbapenemase in carbapenem-resistant Enterobacteriaceae(CRE)isolates.Methods Totally,73 non-reduplicative isolates of CRE were isolated.The expression of carbapenemase gene was identified by polymerase chain reaction(PCR),and the rapid determination of carbapenemase was performed by GICA.PCR was used as reference method to evaluate the accuracy of GICA in the rapid determination of carbapenemase.Results The results of PCR for the 73 CRE isolates showed that there were 51 bla_(KPC) positive isolates(69.9%),19 bla_(NDM) positive isolates(26.0%),2 bla_(IMP) positive isolates(2.7%)and 1 bla_(VIM) positive isolate(1.4%)with no bla_(OXA) positive isolate determined.The accuracy of GICA for the determination of the carbapenemase types of blaKPC,bla_(NDM),bla_(IMP) and bla_(VIM) positive isolates was 100%.The consistency of GICA and PCR in the determination of carbapenemase types of CRE isolates is 100%.Conclusions GICA with the characteristics of simple,rapid and high accuracy has a potential clinical application role in early monitoring of carbapenem-resistant isolates and providing a reference for anti-infection therapy.

关 键 词：耐碳青霉烯类肠杆菌科细菌 碳青霉烯酶 胶体金免疫层析法 聚合酶链反应 快速检测 

分 类 号：R37[医药卫生—病原生物学]