基于线粒体COI序列的矛尾复虾虎鱼5个野生群体的遗传多样性分析  

Genetic diversity based on the mitochondrial COI gene sequences in five wild populations of Synechogobius hasta

作　　者：祝斐[1] 应博凯 贾超峰[1] 孟乾 高波[1] 孙瑞健[1] 宋双双 徐大凤 卢瑶瑶 张志伟[1] 陈淑吟[1] 张志勇[1] 陈心[2] ZHU Fei;YING Bokai;JIA Chaofeng;MENG Qian;GAO Bo;SUN Ruijian;SONG Shuangshuang;XU Dafeng;LU Yaoyao;ZHANG Zhiwei;CHEN Shuyin;ZHANG Zhiyong;CHEN Xin(Jiangsu Marine Fisheries Research Institute,Nantong 226000,China;Laboratory of Life Sciences,Nantong University,Nantong 226000,China)

机构地区：[1]江苏省海洋水产研究所,江苏南通226000 [2]南通大学生命科学学院实验室,江苏南通226000

出　　处：《海洋通报》2021年第6期636-643,共8页Marine Science Bulletin

基　　金：南通市基础科学研究项目(JC2019058);江苏省自然科学基金(BK20181202);江苏省农业科技自主创新资金(CX(17)2021);江苏省农业重大新品种创制项目(PZCZ201744)。

摘　　要：由于近年来过度捕捞及滩涂开发,野生矛尾复虾虎鱼资源量急剧下降。因此,对我国矛尾复虾虎鱼开展遗传多样性研究,有利于掌握矛尾复虾虎鱼种质资源现状。本文基于线粒体COI序列对大连、如东、连云港、长江口和宁波的5个矛尾复虾虎鱼群体开展了遗传多样性研究。结果表明,5个群体中共有17个单倍型,在427个位点中,共检测到15个变异位点,其中简约信息位点11个,单独位点4个。碱基A、T、G、C的平均含量分别为24.6%、29.6%、20.1%、25.7%。5个群体的单倍型多样性和核苷酸多样性分别为0.652和0.00255。野生矛尾复虾虎鱼整体的遗传多样性水平为中等偏下,宁波群体的遗传多样性最高,如东群体最低。群体间遗传分化系数FST及遗传距离分析表明,宁波群体与其他群体遗传距离相对较远,并存在显著的遗传分化;AMOVA分析表明,群体内的遗传变异高于群体间变异(81.45%>18.55%);中性检验结果显示,大连、连云港、长江群体在历史上可能经历过扩张。本研究为野生矛尾复虾虎鱼种质资源保护和合理开发利用提供了理论依据。Overfishing and coastal development in recent years cause the loss of wild Synechogobius hasta population.To study the genetic diversity of different populations of wild Synechogobius hasta,five populations from Dalian,Rudong,Lianyungang,Changjiang River Estuary and Ningbo were analyzed by sequencing the mitochondrial cytochrome oxidase sub-unit I(COI)genes.The results showed that there were 17 haplotypes in 5 populations,and 15 polymorphic sites were detected in 427 bp,including 11 parsimony information sites and 4 single variable sites.The average contents of base A,T,G and C were 24.6%,29.6%,20.1%and 25.7%,respectively.The haplotype diversity and nucleotide diversity of the five populations were 0.652 and 0.00255,respectively.The overall level of genetic diversity is lower than medium.The genetic diversity of Ningbo population is the highest and Rudong population is the lowest.The genetic differentiation index(FST)and genetic dis-tance showed that there was significant genetic differentiation between Ningbo population and other populations,with rela-tively far genetic distance.AMOVA analysis showed that the genetic variation within populations was higher than that among populations(81.45%>18.55%).In terms of the negatively selective neutrality test,the results indicated that Dalian,Lianyun-gang and Changjiang River might have experienced population expansion in history.This study is helpful as a reference for protection of the genetic diversity and breeding of S.hasta.

关 键 词：矛尾复虾虎鱼 遗传多样性 线粒体 COI序列 

分 类 号：P735[天文地球—海洋生物学] Q173[天文地球—海洋科学]