Jagged1在增生性糖尿病视网膜病变纤维血管膜中的表达及其意义  

Expression and significance of Jagged1 in fibrovascular membranes of proliferative diabetic retinopathy

作　　者：郭庆敏[1,4] 孟旭霞 杨堃[2] 余川[3] Guo Qingmin;Meng Xuxia;Yang Kun;Yu Chuan(Department of Ophthalmology,The Affiliated Hospital of Qingdao University,Qingdao 266000,China;Central Lab,The Affiliated Hospital of Qingdao University,Qingdao 266000,China;Department of Ophthalmology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;Hebei Eye Hospital,Hebei Provincial Key Laboratory of Ophthalmology,Hebei Provincial Clinical Research Center for Eye Diseases,Xingtai 054001,China)

机构地区：[1]青岛大学附属医院眼科,266000 [2]青岛大学附属医院中心实验室,266000 [3]郑州大学第一附属医院眼科,450052 [4]河北省眼科医院,河北省眼科学重点实验室,河北省眼部疾病临床医学研究中心,邢台054001

出　　处：《中华实验眼科杂志》2022年第1期29-34,共6页Chinese Journal Of Experimental Ophthalmology

摘　　要：目的观察Notch配体Jagged1在增生性糖尿病视网膜病变(PDR)纤维血管膜中的表达及其意义。方法于2014年7月至2015年7月在青岛大学附属医院眼科收集PDR患者57例60眼玻璃体切割术中纤维血管膜标本。根据术前是否行抗血管内皮生长因子药物玻璃体内注射将患眼分为未注药组30例32眼和注药组27例28眼,其中注药组患眼于手术前2~7 d行雷珠单抗玻璃体内注射。收集同期非糖尿病伴特发性黄斑前膜患者18例18眼黄斑前膜标本为对照组。采用苏木精-伊红染色观察各组标本结构特征;采用免疫组织化学染色法检测未注药组与注药组病理标本中Jagged1、Delta样配体4(Dll4)和Notch1蛋白的表达;采用实时荧光定量PCR测定各组标本中Jagged1、Dll4和Notch1 mRNA相对表达量。采用Pearson线性相关分析评估PDR纤维血管膜标本中Jagged1 mRNA相对表达量与Dll4 mRNA和Notch1 mRNA相对表达量关系。结果光学显微镜下可见PDR纤维血管膜中新生血管生成,且注药组血管管腔狭窄,未注药组血管管腔相对扩张,黄斑前膜中未见新生血管。未注药组和注药组病理标本中均有Jagged1、Dll4和Notch1蛋白表达,主要位于新生血管内皮组织中。未注药组病理标本中Jagged1、Dll4和Notch1蛋白阳性染色吸光度(A)值分别为6.25±1.82、6.87±1.89和5.12±2.14,高于注药组的1.46±0.37、1.55±0.24和1.32±0.53,差异均有统计学意义(t=5.168,P=0.014;t=6.012,P=0.008;t=3.453,P=0.030)。未注药组、注药组和对照组病理标本中Jagged1、Dll4和Notch1 mRNA相对表达量总体比较,差异均有统计学意义(F=77.337、62.305、51.869,均P<0.01);其中,未注药组和注药组病理标本中Jagged1、Dll4和Notch1 mRNA相对表达量均显著高于对照组,注药组病理标本中Jagged1、Dll4和Notch1 mRNA相对表达量均较未注药组明显降低,差异均有统计学意义(均P<0.05)。Jagged1 mRNA相对表达量与Dll4和Notch1 mRNA相对表达量均呈显著正相Objective To observe the expression and significance of Jagged1 in fibrovascular membranes of proliferative diabetic retinopathy(PDR).Methods Sixty preretinal fibrovascular membrane specimens collected from fifty-seven patients(60 eyes)with PDR during vitrectomy in The Affiliated Hospital of Qingdao University from July 2014 to July 2015 were set as the PDR group.The patients were divided into the injection group(30 cases,32 eyes)and non-injection group(27 cases,28 eyes)according to whether they received anti-vascular endothelial factor drug intravitreally before surgery.Ranibizumab injections were administered to the patients in the injection group intravitreally 2-7 days before surgery.Eighteen macular epiretinal membrane specimens obtained from 18 non-diabetic patients were served as the control group.Hematoxylin-eosin staining was applied to observe the structural festures of specimers.The immunohistochemical staining was used to detect the expression levels of Jagged1,Delta-like 4(Dll4)and Notch1 in the injection and non-injection groups,and the real-time fluorescent quantitative polymerase chain reaction was performed to detect the relative expression levels of Jagged1,Dll4 and Notch1 mRNA in the three groups.Pearson linear correlation analysis was used to evaluate the relationships between the expression of Jagged1 mRNA and both Dll4 mRNA or Notch1 mRNA in the PDR fibrovascular membranes.This study protocol adhered to the Declaration of Helsinki and was approved by an Ethics Committee of The Affiliated Hospital of Qingdao University(No.QYFYWZLL25645).Written informed consent was obtained from each patient.Results The neovascularization was found in fibrovascular membranes of PDR with a light microscope,and the lumen of the new blood vessels in the injection group was narrow,but relatively dilated in the non-injection group.There was no neovascularization found in the macular epiretinal membranes.The immunohistochemical staining revealed that there was the positive expression of Jagged1,Dll4 and Notch1 prot

关 键 词：视网膜新生血管 糖尿病视网膜病变 增生性 Jagged1蛋白 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]