SbGA20ox1·SbGA20ox3和SbGA2ox3基因对高粱品种赤霉素表达水平的影响  

Effects of SbGA20ox1,SbGA20ox3 and SbGA2ox3 Genes on the Expression of Gibberellin in Sorghum Varieties

作　　者：商靖 陆劲羽 逄洪波[1] 李雪梅[1] 王艳秋[2] 李玥莹[1] SHANG Jing;LU Jin-yu;PANG Hong-bo(School of Life Sciences,Shenyang Normal University,Shenyang,Liaoning 110034)

机构地区：[1]沈阳师范大学生命科学学院,辽宁沈阳110034 [2]辽宁省农科院,辽宁沈阳110161

出　　处：《安徽农业科学》2022年第2期105-108,共4页Journal of Anhui Agricultural Sciences

基　　金：辽宁省教育厅重点攻关项目(LZD201901);辽宁省教育厅自然科学重点攻关项目(LZD202004);财政部和农业农村部国家现代农业产业技术体系(CARS-06)。

摘　　要：[目的]研究SbGA20ox1、SbGA20ox3和SbGA2ox3基因对高粱品种赤霉素表达水平的影响。[方法]以不同高粱品种为试材(9544.7057为父本与不同母本进行杂交得到子代:辽夏梁1号、辽2297、辽2697、辽5397、01-26B;以及01-26A为母本与不同父本进行杂交得到子代:辽杂35、0-01、9544.7057、3550、辽2697),利用实时荧光定量PCR技术比较不同时期(拔节期、抽穗期、成熟期),不同品种的高粱之间SbGA20ox1基因、SbGA20ox3基因、SbGA2ox3基因表达含量规律。[结果]SbGA20ox1基因在同一父本不同母本高粱组的基因表达规律基本符合抽穗期>成熟期>拔节期;SbGA2ox3基因在同一父本不同母本高粱组的基因表达规律基本符合抽穗期>成熟期>拔节期,SbGA2ox3基因在同一母本不同父本高粱组的基因表达规律基本符合拔节期>成熟期>抽穗期。而SbGA20ox1基因在同一母本不同父本高粱组的基因表达以及SbGA20ox3基因在各品种高粱的基因表达并未发现明显规律,各时期基因的相对表达量大部分品种间无明显差异。[结论]该研究一定程度上揭示了SbGA20ox1、SbGA20ox3和SbGA2ox3基因在多个高粱品种各时期的基因表达规律,为进一步研究调控赤霉素水平的小基因家族的作用机制提供启示。[Objective]To study the effects of SbGA20ox1,SbGA20ox3 and SbGA2ox3 on the expression of gibberellin in sorghum varieties.[Method]Different sorghum varieties were used as test materials(9544.7057 was used as the male parent and different female parents were crossed to obtain offspring:Liaoxialiang 1,Liao 2297,Liao 2697,Liao 5397,01-26B;and 01-26A was used as the female parent crossing with different male parents to obtain offspring:Liaoza 35,0-01,9544.7057,3550,Liao 2697).Real-time fluorescent quantitative PCR technology was used to compare the expression levels of SbGA20ox1,SbGA20ox3,and SbGA2ox3 genes between different periods(joining stage,heading stage,maturity stage)and different varieties of sorghum.[Result]The gene expression pattern of SbGA20ox1 gene in the same male parent and different female parent sorghum groups basically accorded with heading date>mature stage>joining stage;the gene expression law of SbGA2ox3 gene in the same male parent and different female parent sorghum groups basically accorded with heading date>mature stage>joining stage,the gene expression pattern of SbGA2ox3 gene in the same female parent and different male parent sorghum groups basically accorded with jointing stage>mature stage>heading stage.However,the gene expression of SbGA20ox1 gene in the same female parent and different male parent sorghum groups and the gene expression of SbGA20ox3 gene in various varieties of sorghum did not find obvious regularities,and the relative expression levels of genes in each period showed no significant difference among most varieties.[Conclusion]This study revealed to a certain extent the gene expression regularity of SbGA20ox1,SbGA20ox3 and SbGA2ox3 genes in multiple sorghum varieties at various stages,and provided enlightenment for further research on the mechanism of the small gene family that regulates gibberellin levels.

关 键 词：高粱 相对表达量 实时荧光定量 赤霉素 小基因家族 

分 类 号：S188[农业科学—农业基础科学]