基于超临界萃取技术的泰国青柠果皮精油成分解析及其抗肿瘤活性研究  

Component Analysis and Antitumor Activity of Oil from Kaffir Lime Peel Based on Supercritical Fluid Extraction

作　　者：郑莹[1,2,3] 李天娇 包永睿[1,2,3] 王帅 杨欣欣[1,2,3] 赵国斌 孟宪生[1,2,3] ZHENG Ying;LI Tian-jiao;BAO Yong-rui;WANG Shuai;YANG Xin-xin;ZHAO Guo-bin;MENG Xian-sheng(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Multi-dimensional Analysis of Traditional Chinese Medicine Technical Innovation Center,Dalian 116600,China;Liaoning Province Modern Traditional Chinese Medicine Research and Engineering Laboratory,Dalian 116600,China;Dalian Zhuoer Supercritical Technology Development Co.,Ltd.,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药多维分析专业技术创新中心,辽宁大连116600 [3]辽宁省现代中药研究工程实验室,辽宁大连116600 [4]大连卓尔超临界科技发展有限公司,辽宁大连116600

出　　处：《化学试剂》2022年第2期178-185,共8页Chemical Reagents

基　　金：辽宁省中药药效物质组学与作用机理研究创新团队项目(LT2017015)。

摘　　要：主要研究泰国青柠果皮精油成分组成及其抗肿瘤活性。采用超临界二氧化碳萃取技术提取青柠果皮精油,经超高效液相-飞行时间质谱联用仪(UPLC-QTOF-MS)和气相色谱-质谱联用仪(GC-MS)进行成分分析,经与数据库及参考文献比对,鉴定化学成分;采用CCK-8法测定青柠果皮精油抗肝癌细胞株HepG2和结直肠癌细胞株SW620的体外抗肿瘤活性。经UPLC-QTOF-MS指出泰国青柠果皮精油中香豆素类14个、黄酮类12个、萜类6个、鞣质类1个、脂肪酸类1个;经GC-MS指出挥发性成分58个,包括醇类34个、烯烃类12个、酯类5个、醛类2个、芳香烃类2个、醚类2个和酮类1个。细胞实验证明泰国青柠果皮精油对HepG2和SW620细胞株具有不同程度的抑制作用,IC_(50)分别为0.53、0.77 mg/mL,表明其具有较好的抗肿瘤活性。为泰国青柠果皮精油抗肿瘤应用开发提供数据支撑。To study the composition and antitumor activity of oil from Kaffir Lime peel,oil from Kaffir Lime peel was extracted by supercritical carbon dioxide extraction technology.The components were analyzed by UPLC-QTOF-MS and GC-MS.These components were estimated by comparing with database and references.The antitumor activity of oil from Kaffir Lime peel against hepatocellular carcinoma cell line HepG2 and colorectal cancer cell line SW620 in vitro was investigated by CCK-8 assay.The results showed that by using UPLC-QTOF-MS,14 coumarins,12 flavonoids,6 terpenoids,1 tannin and 1 fatty acid were identified in the oil from Kaffir Lime peel.A total of 58 volatile compounds were identified by GC-MS,including 34 alcohols,12 alkenes,5 esters,2 aldehydes,2 aromatic hydrocarbons,2 ethers and 1 ketone.Cell experiments showed that the oil from Kaffir Lime peel had different degree of inhibitory effects on HepG2 and SW620 cell lines,with IC_(50) of 0.53,0.77 mg/mL,respectively,indicating that it had good antitumor activity.This paper provides the data support for the development of antitumor application of oil from Kaffir Lime peel.

关 键 词：泰国青柠果皮精油 超临界流体萃取 抗肿瘤 UPLC-QTOF-MS GC-MS 

分 类 号：R9[医药卫生—药学]