Flesh quality of hybrid grouper(Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)fed with hydrolyzed porcine mucosasupplemented low fishmeal diet  

作　　者：Xuanyi Yang Xinyan Zhi Ziling Song Guanghui Wang Xumin Zhao Shuyan Chi Beiping Tan 

机构地区：[1]Laboratory of Aquatic Nutrition and Feed,College of Fisheries,Guangdong Ocean University,Zhanjiang 524088,China [2]Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang),Zhanjiang 524025,China [3]Aquatic Animals Precision Nutrition and High Efficiency Feed Engineering Research Center of Guangdong Province,Zhanjiang 524088,China [4]Yichang Huatai Biological Technology Co.,Ltd.,Yichang 443500,China

出　　处：《Animal Nutrition》2022年第1期114-124,共11页动物营养（英文版）

基　　金：This study was supported by the Fund of The National Key R&D Program of China(2019YFD0900200);Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)(No.ZJW2019-06);China Agricultural Research System(CARS-47);Guangdong Modern Agriculture Research System-Innovation Team(2020KJ150).

摘　　要：Iso-nitrogenous and iso-lipidic diets containing 0%,3%,6%,9%,and 12%hydrolyzed porcine mucosa(namely,HPM0,HPM3,HPM6,HPM9,and HPM12)were prepared to evaluate their effects on the growth performance,muscle nutrition composition,texture property,and gene expression related to muscle growth of hybrid groupers(Epinephelus fuscoguttatus\Epinephelus lanceolatus_).Groupers were fed to apparent satiation at 08:00 and 16:00 every day for a total of 56 days.It was found that the weight gain percentage in the HPM0,HPM3,and HPM6 groups did not differ(P>0.05).The cooking loss and drip loss of the dorsal muscle in the HPM3 group were lower than those in the HPM6 and HPM9 groups(P<0.05).The hardness and chewiness of the dorsal muscle in the HPM3 group were higher than those in the HPM0,HPM9,and HPM12 groups(P<0.05).The gumminess in the HPM3 group was higher than that in the HPM9 and HPM12 groups(P<0.05).The total essential amino acid content of the dorsal muscle in the HPM12 group was higher than that in the HPM0 group(P<0.05).The contents of total n-3 polyunsaturated fatty acid and total n-3 highly unsaturated fatty acid,as well as the ratio of n-3/n-6 polyunsaturated fatty acid in the dorsal muscle was higher in the HPM0 group than in all other groups(P<0.05).The relative expressions of gene myogenic factor 5,myocyte enhancer factor 2c,myocyte enhancer factor 2a,myosin heavy chain,transforming growth factor-beta 1(TGF-b1),and follistatin(FST)were the highest in the dorsal muscle of the HPM3 group.The results indicated that the growth performance of hybrid grouper fed a diet with 6%HPM and 27%fish meal was as good as that of the HPM0 group.When fish ingested a diet containing 3%HPM,the expression of genes TGF-β1 and FST involved in muscle growth were upregulated,and then the muscle quality related to hardness and chewiness were improved.An appropriate amount of HPM could be better used in grouper feed.

关 键 词：Flesh quality Growth performance Hybrid groupers Hydrolyzed porcine mucosa Muscle growth-related gene 

分 类 号：S965.334[农业科学—水产养殖]