番茄叶霉病菌对氟唑菌酰羟胺敏感基线的建立及氟唑菌酰羟胺田间防病效果评价  

Sensitivity baseline to pydiflumetofen in Passalora fulva and control efficacy of pydiflumetofen against tomato leaf mold

作　　者：李秀环 禾丽菲 李北兴 姜林[2] 刘峰 慕卫 LI Xiuhuan;HE Lifei;LI Beixing;JIANG Lin;LIU Feng;MU Wei(Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,Key Laboratory of Pesticide Toxicology&Application Technique,College of Plant Protection,Shandong Agricultural University,Taian 271018,Shandong Province,China;College of Chemistry and Material Science,Shandong Agricultural University,Taian 271018,Shandong Province,China)

机构地区：[1]山东农业大学植物保护学院/山东省蔬菜病虫生物学重点实验室/山东省高校农药毒理与应用技术重点实验室,山东泰安271018 [2]山东农业大学化工与材料科学学院,山东泰安271018

出　　处：《农药学学报》2022年第1期66-72,共7页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划(2016YFD0200500).

摘　　要：氟唑菌酰羟胺(pydiflumetofen)是一种新型SDHI类杀菌剂,目前在中国仅登记用于防治小麦赤霉病和油菜菌核病,为明确其对番茄叶霉病的防治潜力,测定了番茄叶霉病菌Passalora fulva不同发育阶段以及山东省不同地区采集的菌株对氟唑菌酰羟胺的敏感性,并验证了其田间防治效果。结果表明:氟唑菌酰羟胺对番茄叶霉病菌不同发育阶段均具有较强的抑制活性,其中对孢子萌发和芽管伸长的抑制作用较强,在0.10μg/mL剂量下抑制率均达50%以上。采用菌丝生长速率法测得从山东省8个地区采集分离的103个菌株的EC50值在0.04~1.74μg/mL之间,平均值为(0.67±0.41)μg/mL,其敏感性频率分布呈单峰曲线,可将该平均EC50值作为番茄叶霉病菌对氟唑菌酰羟胺的敏感性基线。两年的田间药效试验结果表明:200 g/L氟唑菌酰羟胺悬浮剂(SC)以有效成分200 g/hm^(2)剂量连续施药两次,距末次施药后7 d对番茄叶霉病的田间防治效果均达80%以上,显著高于对照药剂甲基硫菌灵(thiophanate-methyl)有效成分540 g/hm^(2)和氟吡菌酰胺(fluopyram)有效成分150 g/hm^(2)的防治效果。本研究结果可为氟唑菌酰羟胺在防治番茄叶霉病上应用提供数据支持。Pydiflumetofen is a novel succinate dehydrogenase inhibitor(SDHI),which shows excellent inhibitory activity to many plant pathogens,such as Fusarium graminearum and Sclerotinia sclerotiorum.In this study,the sensitivity to pydiflumetofen of Passalora fulva strains collected from 8 cities in Shandong Province were tested by using mycelial growth rate method.In addition,the control efficacy of pydiflumetofen to tomato leaf mold were also investigated in field.The results showed that pydiflumetofen had strong inhibitory activity on three developmental stages of P.fulva,and the inhibitory activities on spore gemination and germ tube elongation were higher than that on mycelial growth.Inhibition rate of pydiflumetofen to spore gemination and germ tube elongation of P.fulva was over 50%at 0.10μg/mL.The EC50 values frequency distribution of 103 P.fulva isolates to pydiflumetofen on mycelial growth was a unimodal curve and could be regarded as the baseline sensitivity.The EC50 values ranged from 0.04 to 1.74μg/mL,with a mean of(0.67±0.41)μg/mL.In field trials of two years,after the last application at the dose of 200 g a.i./hm^(2),the control efficacy of pydiflumetofen was above 80%,which was significantly higher than that of thiophanate-methyl(540 g a.i./hm^(2))and fluopyram(150 g a.i./hm^(2)).This study provides data support for monitoring the resistance of tomato leaf mold to pydiflumetofen and for efficient controlling tomato leaf mold.

关 键 词：番茄叶霉病菌 氟唑菌酰羟胺 杀菌活性 敏感基线 田间防效 

分 类 号：S482.2[农业科学—农药学]