Porous tantalum-composited gelatin nanoparticles hydrogel integrated with mesenchymal stem cell-derived endothelial cells to construct vascularized tissue in vivo  

作　　者：Zhenhua Zhao Mang Wang Fei Shao Ge Liu Junlei Li Xiaowei Wei Xiuzhi Zhang Jiahui Yang Fang Cao Qiushi Wang Huanan Wang Dewei Zhao 

机构地区：[1]Orthopaedic Department,Affiliated ZhongShan Hospital of Dalian University,No.6 Jiefang Street,Zhongshan District,Dalian,Liaoning 116001,P.R.China [2]National-Local Joint Engineering Laboratory for the Development of Orthopedic Implant Materials,Affiliated ZhongShan Hospital of Dalian University,No.6 Jiefang Street,Zhongshan District,Dalian,Liaoning 116001,P.R.China [3]Key State Laboratory of Fine Chemicals,School of Bioengineering,Dalian University of Technology,No.2,Linggong Road,High-Tech District,Dalian 116024,P.R.China [4]School of Mechanical Engineering,Dalian Jiaotong University,Dalian 116028,P.R.China [5]Reproductive Medicine Centre,Affiliated ZhongShan Hospital of Dalian University,No.6 Jiefang Street,Zhongshan District,Dalian,Liaoning 116001,P.R.China [6]Department of Biomedical Engineering,Faculty of Electronic Information and Electronical Engineering,Dalian University of Technology,Dalian 116024,P.R.China [7]Laboratory Animal Center,Affiliated ZhongShan Hospital of Dalian University,No.6 Jiefang Street,Zhongshan District,Dalian,Liaoning 116001,P.R.China

出　　处：《Regenerative Biomaterials》2021年第6期92-105,共14页再生生物材料（英文版）

基　　金：supported by Postdoctoral Science Foundation of China(No.194012);National Natural Science Foundation of China(No.82172398);Science&Technological Convenience Foundation of Dalian(No.2020JJ27SN076);Doctoral Research Starting Foundation of Affiliated Zhongshan Hospital of Dalian University(No.DLDXZSYY-BK201809).

摘　　要：The ideal scaffold material of angiogenesis should have mechanical strength and provide appropriate physiological microporous structures to mimic the extracellular matrix environment.In this study,we constructed an integrated three-dimensional scaffold material using porous tantalum(pTa),gelatin nanoparticles(GNPs)hydrogel,and seeded with bone marrow mesenchymal stem cells(BMSCs)-derived endothelial cells(ECs)for vascular tissue engineering.The characteristics and biocompatibility of pTa and GNPs hydrogel were evaluated by mechanical testing,scanning electron microscopy,cell counting kit,and live-cell assay.The BMSCs-derived ECs were identified by flow cytometry and angiogenesis assay.BMSCs-derived ECs were seeded on the pTa-GNPs hydrogel scaffold and implanted subcutaneously in nude mice.Four weeks after the operation,the scaffold material was evaluated by histomorphology.The superior biocompatible ability of pTa-GNPs hydrogel scaffold was observed.Our in vivo results suggested that 28 days after implantation,the formation of the stable capillary-like network in scaffold material could be promoted significantly.The novel,integrated pTa-GNPs hydrogel scaffold is biocompatible with the host,and exhibits biomechanical and angiogenic properties.Moreover,combined with BMSCs-derived ECs,it could construct vascular engineered tissue in vivo.This study may provide a basis for applying pTa in bone regeneration and autologous BMSCs in tissue-engineered vascular grafts.

关 键 词：porous tantalum gelatin nanoparticles hydrogel bone marrow mesenchymal stem cell endothelial cell VASCULARIZATION 

分 类 号：R318[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]