Biomimetic three-layered membranes comprising(poly)-e-caprolactone,collagen and mineralized collagen for guided bone regeneration  

作　　者：Jingjing Wu Mengyu Yao Yonggang Zhang Zefeng Lin Wenwu Zou Jiaping Li Pamela Habibovic Chang Du 

机构地区：[1]Department of Biomedical Engineering,School of Materials Science and Engineering,South China University of Technology,Guangzhou 510641,PR China [2]National Engineering Research Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou 510006,PR China [3]Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education,and Innovation Center for Tissue Restoration and Reconstruction,South China University of Technology,Guangzhou 510006,PR China [4]Department of Instructive Biomaterials Engineering,MERLN Institute for Technology-Inspired Regenerative Medicine,Maastricht University,Maastricht 6229 ER,the Netherlands

出　　处：《Regenerative Biomaterials》2021年第6期218-229,共12页再生生物材料（英文版）

基　　金：supported by National Key R&D Program of China(2017YFC1105000);National Natural Science Foundation of China(51572087);Outstanding Scholar Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory(2018GZR110102001);GDSTNWO Science Industry Cooperation Program Chemistry(2018A50501006);the 111 Project(B13039).P.H.and Y.Z.acknowledge the financial support by the Gravitation Program‘Materials Driven Regeneration’,funded by the Netherlands Organization for Scientific Research(NWO)(Grant#024.003.013);J.L.and P.H.acknowledge financial support by the NWO,Applied and Engineering Sciences(NWO-AES,Grant#16711).

摘　　要：The distinct structural properties and osteogenic capacity are important aspects to be taken into account when developing guided bone regeneration membranes.Herein,inspired by the structure and function of natural periosteum,we designed and fabricated using electrospinning a fibrous membrane comprising(poly)-e-caprolactone(PCL),collagen-I(Col)and mineralized Col(MC).The three-layer membranes,having PCL as the outer layer,PCL/Col as the middle layer and PCL/Col/MC in different ratios(5/2.5/2.5(PCM-1);3.3/3.3/3.3(PCM-2);4/4/4(PCM-3)(%,w/w/w))as the inner layer,were produced.The physiochemical properties of the different layers were investigated and a good integration between the layers was observed.The three-layeredmembranes showed tensile properties in the range of those of natural periosteum.Moreover,the membranes exhibited excellent water absorption capability without changes of the thickness.In vitro experiments showed that the inner layer of the membranes supported attachment,proliferation,ingrowth and osteogenic differentiation of human bone marrowderived stromal cells.In particular cells cultured on PCM-2 exhibited a significantly higher expression of osteogenesis-related proteins.The three-layered membranes successfully supported new bone formation inside a critical-size cranial defect in rats,with PCM-3 being the most efficient.The membranes developed here are promising candidates for guided bone regeneration applications.

关 键 词：bone regeneration poly(caprolactone) mineralized collagen electrospinning artificial periosteum 

分 类 号：R318[医药卫生—生物医学工程]