Syndecan-4 functionalization of tissue regeneration scaffolds improves interaction with endothelial progenitor cells  

作　　者：Harleigh Warner Yidi Wu William D.Wagner 

机构地区：[1]Department of Plastic and Reconstructive Surgery,Wake Forest University School of Medicine,Medical Center Blvd.,Winston-Salem,North Carolina 27157,USA [2]Department of Biomedical Engineering,Wake Forest University School of Biomedical Engineering and Sciences,Medical Center Blvd.,Winston-Salem,North Carolina 27157,USA

出　　处：《Regenerative Biomaterials》2021年第6期242-248,共7页再生生物材料（英文版）

基　　金：supported by the Harold S.Geneen Charitable Trust Awards Program for Coronary Heart Disease Research(W.D.W);the Wake Forest School of Medicine Department of Plastic and Reconstructive Surgery.

摘　　要：Key to most implanted cell free scaffolds for tissue regeneration is the ability to sequester and retain undifferentiated mesenchymal stem cells at the repair site.In this report,syndecan-4,a heparan sulfate containing proteoglycan,was investigated as a unique molecule for use in scaffold functionalization.An electrospun hybrid scaffold comprised of poly(glycerol)sebacate(PGS),silk fibroin and type I collagen(PFC)was used as a model scaffold to develop a procedure and test the hypothesis that functionalization would result in increased scaffold binding of endothelial progenitor cells(EPCs).For these studies both Syndecan-4 and stromal derived factor-1a(SDF-1a)were used in functionalization PFC.Syndecan-4 functionalized PFC bound 4.8 fold more SDF-1a compared to nonfunctionalized PFC.Binding was specific as determined by heparin displacement studies.After culture for 7 days,significantly,more EPCs were detected on PFC scaffolds having both syndecan-4 and SDF-1a compared to scaffolds of PFC with only syndecan-4,or PFC adsorbed with SDF-1a,or PFC alone.Taken together,this study demonstrates that EPCs can be bound to and significantly expanded on PFC material through syndecan-4 mediated growth factor binding.Syndecan-4 with a multiplicity of binding sites has the potential to functionalize and expand stem cells on a variety of scaffold materials for use in tissue regeneration.

关 键 词：regenerative scaffolds SYNDECAN-4 stromal derived factor-1a endothelial progenitor cells cardiovascular scaffolds 

分 类 号：R318[医药卫生—生物医学工程]