补肾活血方对DKK1阻断Wnt信号通路后薄型子宫内膜容受性相关蛋白表达的影响  

Effect of Bushen Huoxue Recipe on the expression of thin endometrial tolerance-related proteins after DKK1 blocks the Wnt signaling pathway

作　　者：尹晓丹[1] 杨维[1] 辛明蔚[1] 武颖[1] 何军琴[1] YIN Xiaodan;YANG Wei;XIN Mingwei;WU Ying;HE Jun-qin(Department of TCM,Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University,Beijing 100026,China)

机构地区：[1]首都医科大学附属北京妇产医院中医科,北京100026

出　　处：《中国性科学》2022年第1期124-129,共6页Chinese Journal of Human Sexuality

基　　金：国家自然科学基金项目(82004134);北京中医药科技发展资金项目(QN2018-04)。

摘　　要：目的探讨补肾活血方对DKK1阻断Wnt信号通路后薄型子宫内膜容受性相关蛋白表达的影响。方法建立雌性SD大鼠肾虚血瘀薄型子宫内膜联合模型,将SD大鼠分为对照组、模型组、补佳乐组、补肾活血方高剂量组、补肾活血方中剂量组、补肾活血方低剂量组、Wnt信号通路阻滞剂Dickkopf-1(DKK1)组、DKK1+中药组;各组给予相应的方法干预后,经过大鼠3个动情周期,处死取材。分别检测各组子宫内膜β连环蛋白(β-catenin)、OCT4基因及ABCG2蛋白的表达。结果补肾活血方高剂量组与补佳乐组比较,β-catenin、OCT4基因、ABCG2蛋白的表达差异无统计学意义(P>0.05);DKK1组与模型组比较,差异具有统计学意义(P<0.05);DKK1+中药组与DKK1组比较,差异具有统计学意义(P<0.01)。结论在改善β-catenin、OCT4基因、ABCG2蛋白表达方面,补肾活血方高剂量组能够达到与补佳乐相同的效果;补肾活血方可以增加DKK1阻断Wnt通路后β-catenin、OCT4基因、ABCG2蛋白的表达。Objective To investigate the effect of Bushen Huoxue Recipe on thin endometrial tolerance-related proteins after DKK1 blocks the Wnt signaling pathway. Methods Female SD rats were divided into control group, model group, Bujiale group, Bushen Huoxue Recipe high-dose group, Bushen Huoxue Recipe medium-dose group, Bushen Huoxue Recipe low-dose group, Wnt signaling pathway blocker Dickkopf-1(DKK1) group and DKK1+ Chinese medicine group. Each group was given the corresponding method of intervention and then the rats were killed and extracted after 3 estrus cycles. The expression of β-catenin, OCT4 gene and ABCG2 protein in endometrium of each group were detected. Results There was no significant difference in the expression of β-catenin, OCT4 gene and ABCG2 protein between Bushen Huoxue Recipe high-dose group and Bujiale group(P>0.05). There were statistically significant differences between DKK1 group and model group(P<0.05). The difference between DKK1+ Chinese medicine group and DKK1 group was statistically significant(P<0.01). Conclusions In improving the expression of β-catenin, OCT4 gene and ABCG2 protein, Bushen Huoxue Recipe high-dose group could achieve the same effect as Bujiale;Bushen Huoxue Recipe can increase the expression of β-catenin, OCT4 gene and ABCG2 protein after DKK1 blocks Wnt pathway.

关 键 词：补肾活血方 Wnt/β-catenin信号通路 薄型子宫内膜 子宫内膜干细胞 

分 类 号：R271[医药卫生—中医妇科学]