具有万古霉素高产能的东方拟无枝酸菌诱变株的选育研究  

Screening of Amycolatopsis orientalis Mutation Strain for High Vancomycin Yield

作　　者：陈德刚 牛春 李春玲 CHEN De-gang;NIU Chun;LI Chun-ling(Ningxia Tairui pharmaceutical Company Limited, Yinchuan 750101, China)

机构地区：[1]宁夏泰瑞制药股份有限公司,银川750101

出　　处：《中国兽药杂志》2022年第1期19-26,共8页Chinese Journal of Veterinary Drug

摘　　要：为提高万古霉素(Vancomycin)发酵生产水平,选育出其发酵生产的优良菌株,以东方拟无枝酸菌(Amycolatopsis oriertalsis)菌株V1806作为原始菌株,利用EMS(甲基磺酸乙酯)、UV(紫外诱变)和ARTP(常压室温等离子体诱变)等单一诱变、复合诱变的方法,选育出1株遗传稳定性好、且对万古霉素耐受性强的菌株Vua-15,其中试发酵效价达到7863 mg/L,比原始菌株提高了74.3%。研究结果表明,复合诱变对东方拟无枝酸菌菌种选育的效果优于单一诱变,更易选育出符合预期的优良菌株。研究不仅对万古霉素工业化发酵生产具有重要意义,而且为其他产品发酵菌种的选育提供了一定借鉴。In order to improve the production level of vancomycin fermentation and select the excellent strains for vancomycin fermentation,the strain Amycolatopsis orientalis V1806 was used as the original strain,and was treated with EMS mutation,UV mutation,ARTP mutation and compound mutation,respectively.A strain Vua-15 with stable heredity and strong resistance to vancomycin was screened.The test fermentation titer reached 7863 mg/L,which was 74.3%higher than the original strain.The results showed that the effect of compound mutation on the breeding of Amycolatopsis orientalis was better than that of single mutation,and it was easier to select excellent strains that met the expectation.This study was not only of great significance to the industrial fermentation of vancomycin,but also provided a reference for the breeding of fermentation strains of other products.

关 键 词：万古霉素 高产菌株 诱变 选育 

分 类 号：S859.79[农业科学—临床兽医学]