大腹园蛛重组嵌合蛛丝蛋白:一种高分子量的生物材料  

Araneus ventricosus recombinant chimeric spidroin:a biomaterial with high molecular weight

作　　者：贾秋品 孟清[1] JIA Qiupin;MENG Qing(Institute of Biological Science and Biotechnology,Donghua University,Shanghai 201620,China)

机构地区：[1]东华大学生物科学与技术研究所,上海201620

出　　处：《生物学杂志》2022年第1期51-56,共6页Journal of Biology

基　　金：国家自然科学基金资助项目(31570721);中央高校基本科研业务费专项资金资助项目(CUSF-DH-D-2015045)。

摘　　要：基于大腹园蛛的两种蛛丝基因构建三部分嵌合的重组蛛丝蛋白iN7RC,在大肠杆菌中成功表达,通过纯化得到产量为34 mg/L的高纯度分子质量高达165.2 ku的重组蛛丝蛋白。不同pH值下的圆二色谱分析显示iN7RC蛋白质的二级结构组分中的α-helix结构占比随着pH值的降低明显减少。通过手工牵拉的方式获得具有高强度和高延展性的重组蛛丝纤维,为蛛丝仿生材料的广泛应用奠定基础。A tripartite chimeric recombinant spidroins iN7RC was designed based on two spidroin genes from Araneus ventricosus,suc-cessfully expressed in E.coli cells,and obtained a highly pure 162.5 ku recombinant spidroin through purification at a yield of 34 mg/L.The circular dichroism(CD)spectra of iN7RC spidroin solution under different pHs indicated that the content of the secondary struc-tureα-helix was distinctly decreased along with the declining pH value.Recombinant spider silk fibers with superior strength and ex-tensibility were acquired by hand-drawing,which paved the foundation for the extensive application of biomaterials.

关 键 词：重组蛛丝蛋白 大腹园蛛 蛋白表达 二级结构 蛛丝纤维 

分 类 号：Q78[生物学—分子生物学]