仿生矿化角蛋白多孔材料的制备及性能研究  

Preparation and properties of keratin porous materials with biomimetic mineralization

作　　者：季吉 陈光 李丽丽 袁久刚[1] 苑超 王平[1] 许波 JI Ji;CHEN Guang;LI Lili;YUAN Jiugang;YUAN Chao;WANG Ping;XU Bo(Key Laboratory of Eco-Textile,Ministry of Education,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学生态纺织教育部重点实验室,无锡214122

出　　处：《生物学杂志》2022年第1期57-61,共5页Journal of Biology

基　　金：国家自然科学基金项目(No.31771039);江南大学大学生创新训练计划项目(No.2020214Y);国家重点研发计划项目(No.2017YFB0309200)。

摘　　要：为了解决纯角蛋白多孔材料力学性能差的问题,利用巯基-烯点击化学反应制备聚乙二醇二甲基丙烯酸酯(PEGDMA)改性角蛋白,在此基础上,将改性角蛋白与海藻酸钠(SA)共混得到角蛋白多孔材料,并利用交替矿化法对多孔材料进行仿生矿化,最终制备了仿生矿化角蛋白多孔材料。红外(FTIR)测试显示,PEGDMA成功接枝到角蛋白上,经过改性的角蛋白,其多孔材料弹性得到明显提高。扫描电子显微镜(SEM)、红外以及X射线衍射(XRD)测试结果均显示,HAP成功沉积于多孔材料表面,多孔材料表面形貌变得粗糙。经过矿化,角蛋白多孔材料性能得到全面提升,其屈服点应力达到3.1 MPa,生物相容性提升至117.86%,对Cu^(2+)的吸附量高达159.0 mg/g。这对拓展角蛋白多孔材料的应用具有较好的启示意义。To improve the poor mechanical properties of pure keratin porous materials,a modified keratin of polyethylene glycol dime-thacrylate(PEGDMA)was prepared by thiolene click reaction.The modified keratin was blended with sodium alginate(SA).Finally,biomimetic mineralized keratin porous materials were prepared by alternating mineralization method.FTIR results showed that PEGD-MA was successfully grafted onto keratin.The elasticity of porous materials was obviously improved after modification.The results of SEM,FTIR and XRD showed that HAP was successfully deposited on the surface of porous materials.The surface of porous materials became rough.After mineralization,the properties of keratin porous materials were comprehensively improved,the yield point stress was to 3.1 MPa,the biocompatibility increased to 117.86%,Cu^(2+)adsorption was up to 159.0 mg/g.This was of great significance for expanding the application of keratin porous materials.

关 键 词：角蛋白 仿生矿化 力学性能 生物相容性 吸附 

分 类 号：Q81[生物学—生物工程]