山茱萸环烯醚萜苷对小鼠血管内皮细胞的作用  

Effect of cornus iridoid glycosides on mouse vascular endothelial cells

作　　者：张扬 杨靖[1] 高建萍[2] 陈毅[3] 孔英俊 夏磊磊[3] 张贵锋 李勇超[1] ZHANG Yang;YANG Jing;GAO Jianping;CHEN Yi;KONG Yingjun;XIA Leilei;ZHANG Guifeng;LI Yongchao(School of Life Science and Technology,Henan Institute of Science and Technology,Xinxiang 453003,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,CAS,Beijing 100190,China;Beijing Biosis Healing Biological Technology Co.,Ltd,Beijing 100026,China)

机构地区：[1]河南科技学院生命科技学院,新乡453003 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]北京博辉瑞进生物科技有限公司,北京100026

出　　处：《生物学杂志》2022年第1期75-79,共5页Journal of Biology

基　　金：河南省高等学校重点科研项目计划(17A530002);河南省博士后科研项目(001703078);国家重点研发计划(2018YFA0108200);国家重点研发计划(2018YFC1106400);广东省自然科学基金项目(2014A030312013);海口市海洋经济创新发展示范城市产业链协调创新类项目(HHCL201804)。

摘　　要：对山茱萸环烯醚萜苷类(Cornus iridoid glycosides,CIG)成分进行提取、富集和鉴定,并探究其对血管内皮细胞增殖、形态和凋亡的影响。通过超声提取CIG并经D101大孔树脂去除色素和糖等影响检测的成分,CIG提取物经HPLC-MS检测并与标准品比对和鉴定。通过细胞增殖实验、激光共聚焦显微镜观察和细胞凋亡实验探究CIG对小鼠血管内皮细胞(b.end.3)的影响。结果表明,0.5~50μg/mL CIG可不同程度地促进血管内皮细胞增殖活性,抑制因高糖引起的细胞萎缩和凋亡。山茱萸作为药食同源的中药材可在一定程度上降低血栓形成的风险。The cornus iridoid glycosides(CIG)were extracted and detected,and the influence of CIG to vascular endothelial cell was studied.The cornus was extracted by ultrasonic and treated with D101 macroporous resin to remove the impurities such as pigment and sugar,and the extraction was compared with four standards using HPLC-MS.The effects of CIG on mouse vascular endothelial cell pro-liferation,cell morphology and cell apoptosis were studied.The results showed that,0.5-50μg/mL CIG could promote the vascular endothelial cell proliferation and inhibit cell atrophy and apoptosis caused by high glucose.Cell damage and apoptosis were important for thrombosis.Cornus as the medicine and food could reduce the risk of thrombosis.

关 键 词：山茱萸 环烯醚萜苷 HPLC-MS 血管内皮细胞 细胞凋亡 

分 类 号：Q946[生物学—植物学] R285[医药卫生—中药学]