3D Bioprinted Spheroidal Droplets for Engineering the Heterocellular Coupling between Cardiomyocytes and Cardiac Fibroblasts  

作　　者：Raven El Khoury Naveen Nagiah Joel A.Mudloff Vikram Thakur Munmun Chattopadhyay Binata Joddar 

机构地区：[1]Inspired Materials&Stem-Cell Based Tissue Engineering Laboratory(IMSTEL),The University of Texas at El Paso,El Paso,TX 79968,USA [2]Department of Metallurgical,Materials,and Biomedical Engineering,M201 Engineering,The University of Texas at El Paso,500 W.University Avenue,El Paso,TX 79968,USA [3]Department of Molecular and Translational Medicine,Center of Emphasis in Diabetes and Metabolism,Texas Tech University Health Sciences Center,5001 El Paso Drive,El Paso,TX 79905,USA [4]Border Biomedical Research Center,The University of Texas at El Paso,500 W.University Avenue,El Paso,TX 79968,USA

出　　处：《Cyborg and Bionic Systems》2021年第1期206-221,共16页类生命系统（英文）

基　　金：support from National Institute of General Medical Sciences of the National Institutes of Health under RL5GM118969,TL4GM118971,the NIH BUILD Pilot grant#8UL1GM118970-02.

摘　　要：Since conventional human cardiac two-dimensional(2D)cell culture and multilayered three-dimensional(3D)models fail in recapitulating cellular complexity and possess inferior translational capacity,we designed and developed a high-throughput scalable 3D bioprinted cardiac spheroidal droplet-organoid model with cardiomyocytes and cardiac fibroblasts that can be used for drug screening or regenerative engineering applications.This study helped establish the parameters for bioprinting and cross-linking a gelatin-alginate-based bioink into 3D spheroidal droplets.A flattened disk-like structure developed in prior studies from our laboratory was used as a control.The microstructural and mechanical stability of the 3D spheroidal droplets was assessed and was found to be ideal for a cardiac scaffold.Adult human cardiac fibroblasts and AC16 cardiomyocytes were mixed in the bioink and bioprinted.Live-dead assay and flow cytometry analysis revealed robust biocompatibility of the 3D spheroidal droplets that supported the growth and proliferation of the cardiac cells in the long-term cultures.Moreover,the heterocellular gap junctional coupling between the cardiomyocytes and cardiac fibroblasts further validated the 3D cardiac spheroidal droplet model.

关 键 词：LINKING PRINTING PRINTED 

分 类 号：R54[医药卫生—心血管疾病]