Aerial ammonia exposure induces the perturbation of the interorgan ammonia disposal and branched-chain amino acid catabolism in growing pigs  

作　　者：Shanlong Tang Chang Yin Jingjing Xie Jinglin Jiao Liang Chen Lei Liu Sheng Zhang Hongfu Zhang 

机构地区：[1]State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,100193,China [2]Institute of Biotechnology,Cornell University,Ithaca,NY,14853,USA

出　　处：《Animal Nutrition》2021年第4期947-958,共12页动物营养（英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFD0500501);Agricultural Research Outstanding Talents and Innovation Team(2016-nybrc03);the Fundamental Research Funds for the Central Institute,the Agricultural Science and Technology Innovation Program(ASTIPIAS07)。

摘　　要：Aerial ammonia exposure leads to tissue damage and metabolic dysfunction.However,it is unclear how different organs are coordinated to defend against aerial ammonia exposure.Twenty-four pigs were randomly divided into 4 groups,exposed to 0,10,25 or 35 mg/m^(3) ammonia respectively for 25 d.After above 25 mg/m^(3) ammonia exposure,decreased aspartate(P=0.016),glutamate(P=0.030)and increased ornithine(P=0.002)were found in the ammonia-removing liver,and after high ammonia(35 mg/m^(3))exposure,glutamine synthetase(GS)expression was increased(P=0.012).An increased glutamate(P=0.004)and decreased glutaminase(GLS)expression(P=0.083)were observed in the lungs after high ammonia exposure.There was also an increasing trend of glutamine in the kidneys after high ammonia exposure(P=0.066).For branched-chain amino acid(BCAA)catabolism,high ammonia exposure increased BCAA content in both the lungs and muscle(P<0.05),whereas below 25 mg/m^(3) ammonia exposure increased BCAA only in the lungs(P<0.05).The expression of BCAA transaminase(BCAT1/2)and dehydrogenase complex(BCKDHA/B and DBT)were inhibited to a varying degree in the liver,lungs and muscle after above 25 mg/m^(3) ammonia exposure,especially high ammonia exposure.The expression of BCKDH complex and glutamate-glutamine metabolism-related genes were highly expressed in the liver,followed by the lungs and muscle(P<0.01),whereas the BCAT2 expression was highest in the lungs(P=0.002).Altogether,low ammonia exposure sufficed to evoke the urea cycle to detoxify ammonia in the liver.The process of ammonia removal in the liver and potential ability of the lungs to detoxify ammonia were enhanced with increasing ammonia.Furthermore,high ammonia exposure impaired the BCAA catabolism and decreased the transcripts of the BCAA catabolism-related enzymes,resulting in high BCAA content in extrahepatic tissues.Therefore,with aerial ammonia increasing,an increased urea cycle and glutamine synthesis were ammonia defensive strategies,and high ammonia exposure impaired the BCAA catabolism.

关 键 词：Targeted metabolomics Ammonia exposure Ammonia disposal BCAA catabolism PIG 

分 类 号：S828[农业科学—畜牧学]