Determination of optimal dietary selenium levels by full expression of selenoproteins in various tissues of broilers from 1 to 21 d of age  

作　　者：Xiudong Liao Guoqing Liu Guangming Sun Xiaoming Sun Tao Liu Lin Lu Liyang Zhang Minhong Zhang Yanli Guo Xugang Luo 

机构地区：[1]Mineral Nutrition Research Division,State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing,China [2]Poultry Mineral Nutrition Laboratory,College of Animal Science and Technology,Yangzhou University,Yangzhou,China [3]College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,China [4]Biochemistry Department,Case Western Reserve University,Cleveland,USA [5]State Key Laboratory of Hulless Barley and Yak Germplasm Resources and Genetic Improvement,Lhasa,China [6]Institute of Animal Science and Veterinary,Tibet Academy of Agricultural and Animal Husbandry Sciences,Lhasa,China

出　　处：《Animal Nutrition》2021年第4期1133-1144,共12页动物营养（英文版）

基　　金：supported by the National Natural Science Foundation of China(project no.31601956);the Agricultural Science and Technology Innovation Program(project no.ASTIPIAS09);the China Agriculture Research System of MOF and MARA(project no.CARS-41)。

摘　　要：The current NRC dietary selenium(Se)requirement(0.15 mg/kg)of broilers is primarily based on growth performance data reported in 1986.Our study aimed to determine optimal dietary Se levels of broilers fed a practical corn-soybean meal diet for the full expression of selenoproteins in various tissues.A total of 384 one-d-old male broilers(n=8 replicates/diet)were fed a basal corn-soybean meal diet or the basal diet supplemented with 0.1,0.2,0.3,0.4 or 0.5 mg Se/kg in the form of Na_(2)SeO_(3) for 21 d.Regression analysis was conducted to evaluate the optimal dietary Se levels using broken-line,quadratic or asymptotic models.The activity of glutathione peroxidase(GPX)in the plasma,liver,kidney and pancreas,iodothyronine deiodinase(DIO)in the plasma,liver and pancreas,and thioredoxin reductase(Txnrd)in the liver and pancreas,the mRNA levels of Gpx1,Gpx4,Dio1,selenoprotein(Seleno)h,Selenop and Selenou in the liver,Gpx4,Dio1,Txnrd1,Txnrd2,Selenoh,Selenop and Selenou in the kidney,and Gpx1,Gpx4,Selenoh and Selenou in the pancreas,and the protein levels of GPX4 in the liver and kidney of broilers were influenced(P<0.05)by added Se levels,and increased quadratically(P<0.05)with the increase of added Se levels.The estimates of optimal dietary Se levels were 0.07 to 0.36 mg/kg based on the fitted broken-line,quadratic or asymptotic models(P<0.001)of the aforementioned selenoprotein expression in the plasma,liver and kidney,and 0.09 to 0.46 mg/kg based on the fitted broken-line models(P<0.001)of the aforementioned selenoprotein expression in the pancreas.The results indicate that the optimal dietary Se levels would be 0.36 mg/kg to support the full expression of selenoproteins in the plasma,liver and kidney,and 0.46 mg/kg to support the full expression of selenoproteins in the pancreas of broilers fed a practical corn-soybean meal diet from 1 to 21 d of age.

关 键 词：SELENIUM SELENOPROTEIN Gene expression Requirement BROILER 

分 类 号：S831.5[农业科学—畜牧学]