Serum trimethylamine-N-oxide and gut microbiome alterations are associated with cholesterol deposition in the liver of laying hens fed with rapeseed meal  

作　　者：Liping Zhu Jianping Wang Xuemei Ding Shiping Bai Qiufeng Zeng Yue Xuan Gregory S.Fraley Keying Zhang 

机构地区：[1]Animal Nutrition Institute,Key Laboratory for Animal Disease-Resistance Nutrition of China,Ministry of Education,Sichuan Agricultural University,Chengdu,Sichuan,611130,China [2]Department of Biology,Hope College,Holland,MI,49423,USA

出　　处：《Animal Nutrition》2021年第4期1258-1270,共13页动物营养（英文版）

基　　金：fanatically supported by National Science and Technology Project(2014BAD13B04);the Sichuan Provincial Science and Technology project(2018NZ20009,2014NZ0043)。

摘　　要：Sinapine derived from cruciferous plants could be converted into trimethylamine by intestinal microbiota.Its metabolite,trimethylamine N-oxide(TMAO),is closely linked to increased risk of cardiovascular disease and fat deposition in mammals.Hens fed with rapeseed meal(RSM)suffered from fatty liver hemorrhage syndrome(FLHS).This study was conducted to investigate whether RSM-induced fatty liver is due to TMAO via altering microbiota composition and diversity.At 33 weeks of age,600 laying hens were randomly divided into 5 treatment groups,namely control and 14%RSM treatment groups(DY5,with 16.2% erucic acid [EA]and 74.66% glucosinolate[Gl]contents;MB1,with 3.50% EA and 43.23 %Gl contents;DY6,with 6.7% EA and 22.67% Gl contents;XH3,with 44.60% EA and 132.83% Gl contents)for 8 weeks.Results revealed that 3 hens died due to liver hemorrhage after ingesting 14% RSM diet.The 14% RSM decreased serum low-density lipoprotein cholesterol(LDL-C)content(P<0.01)while tended to increase serum TMAO content compared to the control group(P=0.08).The 14% RSM diet increased red oil O optical density(P<0.01),and increased total cholesterol(TC)and LDL-C content in the liver(P<0.01,and P<0.01,respectively).The 14%RSM decreased liver total bile acid(TBA)content compared to the control(P<0.01).The DY6 had a higher TBA content in the liver than the XH3(P<0.01).The 14%RSM decreased mRNA abundance of liver X receptors alpha(LXR-α,P=0.01),and increased mRNA abundance of sterol response element binding protein 2(SREBP-2,P=0.04).Results revealed that the in-feed RSM could alter richness and diversity of cecal microbiota compared to the control(P<0.05).Liver TC content and serum TMAO showed a negative relationship with Proteobacteria and Actinobacteria(P=0.04).In conclusion,14%RSM increased liver TC and induced high liver score of FLHS,which was possibly associated with the altered cecal microbiota composition,increased serum TMAO levels and LXR-αand SREBP-2 expressions.

关 键 词：SINAPINE Expeller rapeseed meal Trimethylamine N-oxide Liver lipid Cecum microbiota Laying hen 

分 类 号：S831[农业科学—畜牧学]