Whole transcriptome analysis of RNA expression profiles reveals the potential regulating action of long noncoding RNA in lactating cows fed a high concentrate diet  

作　　者：Qu Chen Chen Wu Zhihao Yao Liuping Cai Yingdong Ni Shengyong Mao Ruqian Zhao 

机构地区：[1]Key Laboratory of Animal Physiology&Biochemistry,Nanjing Agricultural University,Nanjing 210095,China [2]Laboratory of Gastrointestinal Microbiology,Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China

出　　处：《Animal Nutrition》2021年第4期1315-1328,共14页动物营养（英文版）

基　　金：the financial contributions of the Fundamental Research Funds for the Central Universities(JCQY201905);National Key Research and Development Project(2016YFD0501203)。

摘　　要：Subacute ruminal acidosis(SARA)is a common metabolic disease in the dairy farming industry which is usually caused by an excessive amount of high concentrate diet.SARA not only threatens animal welfare but also leads to economic losses in the farming industry.The liver plays an important role in the distribution of nutritional substances and metabolism;however,a high concentrate diet can cause hepatic metabolic disorders and liver injury.Recently,noncoding RNA has been considered as a critical regulator of hepatic disease,however,its role in the bovine liver is limited.In this study,12 mid-lactating dairy cows were randomly assigned to a control(CON)group(40% concentrate of dry matter,n=6)and a SARA group(60% concentrate of dry matter,n=6).After 21 d of treatment,all cows were sacrificed,and liver tissue samples were collected.Three dairy cows were randomly selected from the CON and SARA groups respectively to perform whole transcriptome analysis.More than 20,000 messenger RNA(mRNA),10,000 long noncoding RNA(lncRNA),3,500 circular RNA(circRNA)and 1,000 micro RNA(miRNA)were identified.Furthermore,43 mRNA,121 lncRNA and 3 miRNA were differentially expressed,whereas no obvious differentially expressed circRNA were detected between the 2 groups.Gene Ontology(GO)annotation revealed that the differentially expressed genes were mainly enriched in oxidoreductase activity,stress,metabolism,the immune response,cell apoptosis,and cell proliferation.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis showed that the deferentially expressed genes were highly enriched in the phosphatidylinositol 3 kinase(PI3) K-serine/threonine kinase(AKT)signaling pathway(P<0.05).According to KEGG pathway analysis,the differentially expressed lncRNA(DElncRNA)target genes were mainly related to proteasomes,peroxisomes,and the hypoxia-inducible factor-1 signaling pathway(P<0.005).Further bioinformatics and integrative analyses revealed that the lncRNA were strongly correlated with mRNA;therefore,it is reasonable to speculate that l

关 键 词：Whole transcriptome sequencing Liver High concentrate diet Dairy cow 

分 类 号：S823.5[农业科学—畜牧学]