土壤盐胁迫对日本荚蒾生理生化特性的影响  

Effect of Different Salt Stress on Physiological and Biochemical Properties of Viburnum japonicum

作　　者：徐千瑞 周星怡 李贺鹏[3] 岳春雷[3] XU Qian-rui;ZHOU Xing-yi;LI He-peng;YUE Chun-lei(School of Forestry and Bio-technology,Zhejiang A&F University,Hangzhou 311300,China;School of Life Sciences,Nanjing Agricultural University,Nanjing 210095,China;Zhejiang Academy of Forestry,Hangzhou 310023,China)

机构地区：[1]浙江农林大学林业与生物技术学院,浙江杭州318020 [2]南京农业大学生命科学学院,江苏南京210095 [3]浙江省林业科学研究院,浙江杭州310023

出　　处：《浙江林业科技》2022年第1期24-30,共7页Journal of Zhejiang Forestry Science and Technology

基　　金：浙江省省院合作项目(2015SY01);浙江省院所专项项目(2016F30011)。

摘　　要：为评价日本荚蒾Viburnum japonicum的耐盐性,以日本荚蒾3年生实生苗为试验材料进行盆栽盐胁迫试验,设置土壤含盐量分别为0(CK)、0.15%、0.30%、0.45%、0.60%和0.75%共6个浓度的土壤盐分处理,分别在盐胁迫10 d、20 d、30 d、40 d时测定植株叶片的丙二醛(MDA)含量、抗氧化酶活性及渗透调节物质含量等生理生化指标。结果表明,植株叶片的MDA含量、可溶性蛋白含量及可溶性糖含量与土壤含盐量成正比;随着盐胁迫时间的延长,0.15%土壤含盐量处理组植株叶片的超氧化物歧化酶(SOD)活性呈上升趋势,CK与其他处理组植株叶片的SOD活性均呈先上升后下降趋势;除0.60%及0.75%含盐量处理组外,其余各处理组植株叶片的过氧化物酶(POD)活性均高于CK的,但随着胁迫的持续进行,0.45%土壤含盐量处理组植株叶片的POD活性持续下降,CK、0.15%及0.30%土壤含盐量处理组植株叶片的POD活性在上升一段时间后呈下降趋势。综上所述,日本荚蒾对轻度土壤盐胁迫(0.15%及以下土壤含盐量)具有一定的抵抗能力和适应能力,但在0.30%及以上含盐量土壤条件下无法长期存活。以上研究结果为极小种群植物日本荚蒾的保护、迁地引种及园林推广提供了科学依据。On June 2020, 3-year Viburnum japonicum seedlings were potted for experiment. On July 13 th 2020, potted seedlings were treated by 0(CK), 0.15%, 0.30%, 0.45%, 0.60% and 0.75% Nacl solution. Physiochemical indicators were measured 10 d, 20 d, 30 d, 40 d later. The results showed that the content of MDA, soluble protein and soluble sugar of the plant leaves increased with soil salt content. With the duration of salt stress,the activity of superoxide dismutase(SOD) of seedlings of treatment with 0.15% showed an upward trend, while that of CK and the other treatments increased first and then decreased. Peroxidase(POD) activity of treated seedlings was higher than that of CK, except that treated by 0.60% and 0.75%.But with the duration of stress, POD activity of the treatment with 0.45% continued to decrease, while that of CK and with 0.15% and 0.30%increased first and decreased later. The experiment demonstrated that V. japonicum had resistance and adaptability to soil salt content of 0.15% and below, but cannot survive for a long time under salt content of 0.30% and above.

关 键 词：日本荚蒾 盐胁迫 耐盐性 生理生化特性 

分 类 号：S794.4[农业科学—林木遗传育种]