EB病毒转化的永生化B细胞系的建立及应用进展  

Establishment and application of immortalized B cell lines transformed by Epstein Barr virus

作　　者：李艳娜 夏妍[1] 张淑云[1] Yanna Li;Yan Xia;Shuyun Zhang(Department of Clinical Laboratory,Scientic Research Center,the Second Affiliated Hospital of Harbin Mebical University,Harbin 150086,China)

机构地区：[1]哈尔滨医科大学附属第二医院科研实验中心,150086

出　　处：《国际免疫学杂志》2021年第6期683-688,共6页International Journal of Immunology

基　　金：吴阶平医学基金会临床科研专项资助基金(320.6750.18230)。

摘　　要：人外周血B细胞来源于骨髓,是异质性的。EB病毒(Epstein-Barr virus,EBV)可在体外感染静止B细胞(naive B cells),利用其潜伏基因表达产物改变B细胞生命周期,从而形成永生化B淋巴母细胞系(B lymphoblastoid cell lines,B-LCLs),是近年来常用的B细胞永生化的有效方法。B-LCLs常被用作外周血B细胞的替代物和DNA的资源库,通过其单克隆抗体分泌功能、抗原提呈作用和相对稳定的遗传背景等广泛应用于生命科学的各个领域。Human peripheral blood B cells are derived from bone marrow,which are heterogeneous.Epstein-Barr virus(EBV)can infect naive B cells in vitro and use its latent gene expression products to change the life cycle of B cells,thus forming immortalized B lymphoid cell lines(B-LCLs),which is an effective method of immortalization of B cells in recent years.B-LCLs is often used as a substitute for peripheral blood B cells and a resource bank for DNA.It is widely used in various fields of life science through its antigen presentation function secreted by monoclonal antibodies and relatively stable genetic background.

关 键 词：EB病毒 B细胞 B淋巴母细胞系 单克隆抗体 抗原提呈 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]