血细胞分离机不同采集程序采集儿童自体外周血造血干细胞效果观察  

Effect observation of different collection procedures of blood cell separator on collection of autologous peripheral blood hematopoietic stem cells in children

作　　者：徐鸥 胡绍燕[1] Xu Ou;Hu Shaoyan(Department of Hematology,Children's Hospital of Soochow University,Suzhou 215000,China)

机构地区：[1]苏州大学附属儿童医院血液科,江苏苏州215000

出　　处：《白血病．淋巴瘤》2021年第12期744-747,共4页Journal of Leukemia & Lymphoma

摘　　要：目的探讨COBE Spectra血细胞分离机不同采集程序采集儿童自体外周血造血干细胞的效果。方法回顾性分析苏州大学附属儿童医院2018年7月至2020年1月采用COBE Spectra血细胞分离机单个核细胞(MNC)或全自动外周血干细胞(AutoPBSC)程序采集自体外周血造血干细胞的10例患儿临床资料,年龄3~10岁,体质量15~31 kg。MNC组5例,AutoPBSC组5例。结果共采集自体外周血造血干细胞25次,平均采集2.5次(1~4次),MNC组采集10次,AutoPBSC组采集15次。采集前干细胞数[中位数(范围)]MNC组为19.3/μl(3.5~129.0/μl),AutoPBSC组为9.4/μl(2.2~38.7/μl);单次采集获得CD34+细胞数MCN组为1.22×10^(6)/kg(0.18×10^(6)/kg~6.30×10^(6)/kg),AutoPBSC组为0.85×10^(6)/kg(0.13×10^(6)/kg~2.64×10^(6)/kg)。相关性分析显示,采集获得CD34+细胞数和采集前干细胞数呈正相关(AutoPBSC组:r=0.921,P<0.01;MNC组:r=0.833,P=0.003)。采集效率MNC组为5.4%(3.4%~11.2%),AutoPBSC组为10.4%(4.7%~13.9%),差异有统计学意义(Z=2.163,P=0.031)。结论COBE Spectra血细胞分离机AutoPBSC程序采集儿童自体外周血造血干细胞的效果优于MNC程序。Objective To investigate the effect of different collection procedures of COBE Spectra blood cell separator on collection of autologous peripheral blood hematopoietic stem cells in children.Methods The clinical data of 10 children who were collected autologous peripheral blood hematopoietic stem cells with the monocytes(MNC)or fully automatic peripheral blood stem cells(AutoPBSC)programs of COBE Spectra blood cell separator in the Children's Hospital of Soochow University from July 2018 to January 2020 were retrospectively analyzed,and the children were 3-10 years old with the body weight of 15-31kg.There were 5 cases in MNC group and 5 cases in AutoPBSC group.Results Autologous peripheral blood hematopoietic stem cells were collected for 25 times,with an average of 2.5 times(1-4 times),10 times in MNC group and 15 times in AutoPBSC group.The number of stem cells[the median(the range)]before collection was 19.3/μl(3.5-129.0/μl)in MNC group and 9.4/μl(2.2-38.7/μl)in AutoPBSC group.The number of CD34+cells of single collection was 1.22×10^(6)/kg(0.18×10^(6)/kg-6.30×10^(6)/kg)in MCN group and 0.85×10^(6)/kg(0.13×10^(6)/kg-2.64×10^(6)/kg)in AutoPBSC group.Correlation analysis showed that there was a positive correlation between the number of collected CD34+cells and the number of stem cells before collection(AutoPBSC group:r=0.921,P<0.01;MNC group:r=0.833,P=0.003).The collection efficiency was 5.4%(3.4%-11.2%)in MNC group and 10.4%(4.7%-13.9%)in AutoPBSC group,and the difference was statistically significant(Z=2.163,P=0.031).Conclusion The collection effect of children's autologous peripheral blood hematopoietic stem cells with COBE Spectra blood cell separator AutoPBSC program is better than that with MNC program.

关 键 词：儿童 外周血造血干细胞 COBE Spectra血细胞分离机 采集程序 

分 类 号：R457.7[医药卫生—治疗学]