广东省凤尾蕨属植物抗白血病药物研究  被引量:2

Anti-Leukemia Effect of Pteris Plants from Guangdong Province

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作  者:曾德贵[1] 冯东辉 ZENG Degui;FENG Donghui(Shenzhen Center for Chronic Disease Prevention and Control,Shenzhen,Guangdong,China 518020;Shenzhen Hospital of University of Chinese Academy of Sciences,Shenzhen,Guangdong,China 518107)

机构地区:[1]广东省深圳市慢性病防治中心,广东深圳518020 [2]中国科学院大学深圳医院,广东深圳518107

出  处:《中国药业》2022年第3期41-43,共3页China Pharmaceuticals

摘  要:目的探讨广东地区5种常见凤尾蕨属植物半边旗、剑叶凤尾蕨、刺齿凤尾蕨、蜈蚣草和井栏边草对白血病细胞生长的抑制作用。方法用乙醇经索氏提取器分别提取5种植物的有效成分,以二甲基亚砜分别配制供试品溶液;将供试品溶液加入对数生长期的人早幼粒白血病HL-60细胞株(简称HL-60细胞)和人慢性髓样白血病K562细胞株(简称K562细胞)中,采用台盼蓝染色法染色,统计细胞存活率。结果半边旗、剑叶凤尾蕨、刺齿凤尾蕨、蜈蚣草和井栏边草对HL-60细胞和K562细胞的生长均有抑制作用,其中半边旗和刺齿凤尾蕨对HL-60细胞生长的抑制作用较强,4.0 g/L半边旗和刺齿凤尾蕨乙醇提取物中,HL-60细胞的存活率均为0;半边旗和蜈蚣草对K562细胞生长的抑制作用较强,4.0 g/L半边旗和蜈蚣草乙醇提取物中,K562细胞的存活率分别为0和7.70%。结论 5种凤尾蕨属植物对HL-60细胞的生长抑制作用强于K562细胞;除半边旗外,刺齿凤尾蕨和蜈蚣草可能是潜在的抗白血病药物。Objective To investigate the inhibitory effects of five common Pteris plants including Pteris semipinnata,Pteris e nsiformis,Pteris dispar,Pteris vittata and Pteris multifida from Guangdong Province on the growth of leukemia cell lines.Methods The active ingredients from five Pteris plants were extracted with ethanol by the Soxhlet’s extractor,and the test solution was prepared with dimethyl sulfoxide(DMSO).The test solution was added to the human promyelocytic leukemia HL-60 cell line(hereinafter referred to as HL-60 cells) and human chronic myeloid leukemia K562 cell line(hereinafter referred to as K562 cells) during the logarithmic growth stage,and the cell survival rate was counted by the trypan blue staining.Results Pteris semipinnata,Pteris ensiformis,Pteris dispar,Pteris vittata and Pteris multifida could inhibit the growth of HL-60 cells and K562 cells.Among them,Pteris semipinnata and Pteris dispar showed the stronger inhibition on the growth of HL-60 cells,and the survival rate of HL-60 cells was zero in 4.0 g/L of ethanol extracts of Pteris semipinnata and Pteris dispar.Pteris semipinnata and Pteris vittata showed the stronger inhibition on the growth of K562 cells,and the survival rate of K562 cells was zero in 4.0 g/L of ethanol extracts of Pteris semipinnata and Pteris vittata.Conclusion The inhibitory effect of five Pteris plants on the growth of HL-60 cells is stronger than that on the growth of K562 cells.In addition to the Pteris semipinnata,Pteris dispar and Pteris vittata may be potential anti-leukemia herbs.

关 键 词:半边旗 剑叶凤尾蕨 刺齿凤尾蕨 蜈蚣草 井栏边草 人早幼粒白血病HL-60细胞株 人慢性髓样白血病K562细胞株 

分 类 号:R932[医药卫生—生药学] R285.5[医药卫生—药学]

 

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