莲雾果实采后木质素合成相关MYB基因家族及其作用分析  

Lignin Synthesis Related MYB Gene Family and the Function Analysis of Postharvest Wax Apple Fruits

作　　者：聂珂 匡凤元 张珅 吴光斌 陈发河 NIE Ke;KUANG Feng-yuan;ZHANG Shen;WU Guang-bin;CHEN Fa-he(College of Food and Biological Engineering,Jimei University,Xiamen 361000,China)

机构地区：[1]集美大学食品与生物工程学院,福建厦门361000

出　　处：《保鲜与加工》2022年第1期92-101,109,共11页Storage and Process

基　　金：国家自然科学基金面上项目(31171777);福建省自然科学基金项目(2018J01448)。

摘　　要：MYB转录因子在植物苯丙烷代谢的调控中具有重要调节作用,对木质素的合成有影响。为解析MYB基因家族在调控采后莲雾果实絮状绵软的分子机制,利用莲雾转录组数据库测序结果和生物信息学方法,对莲雾果实木质素合成相关的20个MYB转录因子基因(MYB基因)的理化性质和结构功能进行预测,并选择在贮藏期间差异表达的1个基因进行克隆和表达,分析其对莲雾果实木质素合成的影响。生物信息学分析表明:20个MYB基因包含91~591个不等的氨基酸残基,定位于细胞核,不存在跨膜结构;二级结构主要由α-螺旋和随机卷曲组成,在所有莲雾果实MYB蛋白中均有分布,20条莲雾MYB蛋白的三级结构绝大部分相似。系统发育树结果表明,莲雾MYB蛋白与番樱桃、巨桉和玫瑰木的亲缘性较近。从莲雾果实中成功克隆得到SsMYB48基因,GenBank登录号为MK204557.1;拟南芥的组织表达结果表明,SsMYB48基因对木质素合成关键酶基因AtF5H和AtPOD的影响较大,说明SsMYB48基因对木质素的合成可能起抑制作用。该研究结果为莲雾MYB基因家族功能的进一步研究提供了理论依据。MYB transcri ption factor plays an important role in the regulation of phenylpropane metabolism in plants, and affects lignin synthesis. In order to analyze the molecular mechanism of MYB gene family in regulating the cottony softening of postharvest wax apple fruits, the physicochemical properties and structural functions of20 lignin synthesis related MYB transcription factor genes in wax apple fruits were predicted using the sequencing results of wax apple transcriptome database and bioinformatics methods, and a differentially expressed gene during storage was cloned and expressed to analyze the effects on lignin synthesis in wax apple fruits. Bioinformatics analysis showed that 20 MYB genes contained 91 ~591 amino acid residues, which were located in the nucleus without transmembrane structure. The secondary structure was mainly composed of α-helix and random coil, which were distributed in all MYB proteins. Besides, the tertiary structures of these 20 MYB proteins were mostly similar. Phylogenetic tree results showed that MYB proteins were closely related to Syzygium oleosum, Eucalyptus grandis, and Rhodamnia argentea. The SsMYB48 gene was successfully cloned from wax apple with the GenBank accession number of MK204557.1. The tissue expression res ults on Arabidopsis thaliana showed that the SsMYB48 gene presented a great influence on the lignin synthesis related key enzymes genes(AtF5 H and AtPOD), which indicated that the SsMYB48 gene might inhibit the synthesis of lignin. These results could provide a theoretical basis for the further study of the MYB gene family functions in wax apple.

关 键 词：莲雾 MYB转录因子家族 木质素 生物信息学 表达分析 

分 类 号：S667.9[农业科学—果树学]