Expression, secretion and functional characterization of three laccases in E. coli  

作　　者：Yating Mo Hou Ip Lao Sau Wa Au Ieng Chon Li Jeremy Hu Hoi Man Yuen Wai Man Cheong Owen Lok In Lo Leo Chi U.Seak 

机构地区：[1]Pui Ching Middle School(Macao),Edificio Pui Ching,7A Av.de Horta e Costa,Macao SAR,China [2]Department of Physiology,Development of Neuroscience,University of Cambridge,Cambridge,CB23DY,United Kingdom [3]The Centre for Inflammation Research,University of Edinburgh,47 Little France Crescent,Edinburgh,EH164TJ,United Kingdom

出　　处：《Synthetic and Systems Biotechnology》2022年第1期474-480,共7页合成和系统生物技术（英文）

摘　　要：Endocrine Disrupting Chemicals(EDCs)are a group of molecules that can influence hormonal balance,causing disturbance of the reproductive system and other health problems.Despite the efforts to eliminate EDC in the environment,all current approaches are inefficient and expensive.In previous research,studies revealed that laccase-producing microorganisms may be a potential candidate for EDC degradation,as laccases have been found to be able to degrade many kinds of EDCs effectively and steadily.Here,we created two recombinant laccases,each fused with secretion peptide,Novel Signal Peptide 4(NSP4),and expressed them in Escherichia coli(E.coli,BL21),together with one laccase without secretion peptide.We first optimized the culture condition of expressing these laccases.Then,we test the activity of the recombinant laccases of decolorizing of a synthetic dye,indigo carmine.Finally,we confirmed the secreted can degrade one of the EDCs,β-estradiol,showing the potential of using the laccase secretion system to degrade toxic compounds.

关 键 词：LACCASE Endocrine disrupting chemicals(EDCs) 

分 类 号：Q93[生物学—微生物学]