基于DNA损伤与修复抑制初探银杏叶片对燃煤污染型砷中毒患者肝损伤的影响  

Effects of ginkgo biloba tablets on liver injury in patients with coal-burning-borne arsenism based on DNA damage and repair inhibition

作　　者：魏绍峰[1] 孙宝飞[1] 邹忠兰 罗鹏[1] 张爱华[1] Wei Shaofeng;Sun Baofei;Zou Zhonglan;Luo Peng;Zhang Aihua(Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,School of Public Health,Guizhou Medical University,Guiyang 550025,China)

机构地区：[1]贵州医科大学环境污染与疾病监控教育部重点实验室公共卫生与健康学院,贵阳550025

出　　处：《中华地方病学杂志》2022年第1期7-13,共7页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81430077、U1812403、81760572)。

摘　　要：目的探讨DNA损伤和修复抑制在银杏叶片对燃煤污染型砷中毒患者肝损伤影响中的作用。方法2017年3月在贵州省兴仁县雨樟镇交乐村砷中毒病区,按照《地方性砷中毒诊断》(WS/T 211-2015)标准和《职业性中毒性肝病诊断标准》(GBZ 59-2010),筛选出52例砷中毒患者作为银杏叶片干预组,49例砷中毒患者作为干预对照组。银杏叶片按照临床常用方法给药,口服3个月(1片/次,3次/d),所有对象干预期间未给予其他药物,干预对照组给予安慰剂,方法同银杏叶片干预组。选择12 km以外非燃用高砷煤、临床检测无肝功能异常的41例居民作为正常对照组。干预前和3个月干预结束时进行体检。在获得本人知情同意并签署知情同意书的情况下,收集晨尿及外周静脉血,分别采用电感耦合等离子体质谱仪(ICP-MS)检测尿砷含量,全自动生化分析仪检测肝功能生化指标[白蛋白(ALB)、白蛋白/球蛋白比值(A/G)、胆碱酯酶(CHE)、总胆汁酸(TBA)],单细胞凝胶电泳实验检测DNA损伤情况,荧光定量PCR法检测miR-145(修复抑制指标)的表达。结果共纳入研究对象116人,正常对照组41人、银杏叶片干预组39人、干预对照组36人,银杏叶片干预组和干预对照组在年龄、性别比例、吸烟习惯及饮酒方面与正常对照组比较差异均无统计学意义(P均>0.05)。干预前银杏叶片干预组尿砷含量、TBA水平、DNA损伤程度[彗星尾DNA百分含量(TailDNA%)和彗星尾矩(OTM)]、血浆miR-145表达水平[(38.75±19.09)μg/g Cr、(11.13±1.55)μmol/L、8.50±0.88、7.43±0.68、5.78±0.75]均高于正常对照组[(11.62±5.33)μg/g Cr、(5.36±0.87)μmol/L、5.24±0.33、4.71±0.29、2.05±0.27],差异均有统计学意义(P均<0.05);ALB、A/G和CHE水平均低于正常对照组,差异均有统计学意义(P均<0.05)。干预后,银杏叶片干预组尿砷含量、TBA水平、DNA损伤程度(TailDNA%和OTM)、血浆miR-145表达水平均低于干预前,差异�Objective To investigate the role of DNA damage and repair inhibition in the effect of ginkgo biloba on liver injury in patients with coal-burning-borne arsenism.Methods In March 2017,the investigation was conducted in Jiaole village arsenic poisoning area in Yuzhang Town,Xingren County,Guizhou Province.According to the"Diagnosis of Endemic Arsenicosis"(WS/T 211-2015)and the"Diagnostic Criteria of Occupational Toxic Hepatopathy"(GBZ 59-2010),52 patients with arsenism were selected as the ginkgo biloba intervention group,and 49 cases of arsenism patients as intervention control group.Ginkgo biloba tablets were given orally for 3 months(1 tablet/time,3 times/d)according to the commonly used clinical methods,and no other drugs were given to all subjects during the intervention period.The intervention control group was given placebo in the same way as that of ginkgo biloba intervention group.A total of 41 residents who did not burn high arsenic coal 12 km away with no abnormal liver function were selected as normal control group.Physical examinations were performed before the intervention and at the end of the intervention at 3 months.After receiving signed informed consent,morning urine and peripheral venous blood samples were collected to detect urinary arsenic content by inductively coupled plasma mass spectrometry(ICP-MS);liver function biochemical indexes[albumin(ALB),albumin/globulin(A/G),cholinesterase(CHE),total bile acid(TBA)]were determined by automatic biochemical analyzer,DNA damage by single-cell gel electrophoresis assay,and the expression of miR-145(repair inhibition index)by qRT-PCR.Results There were 116 subjects,41 in normal control group,39 in ginkgo biloba intervention group and 36 in intervention control group.In ginkgo biloba and intervention and intervention control groups,there was no significant difference in age,gender,smoking habits and drinking compared with normal control group(P>0.05).Urinary arsenic content,TBA level,DNA damage degree[comet tail DNA percentage(TailDNA%)and olive tail mo

关 键 词：砷中毒 煤 肝 MIR-145 银杏 

分 类 号：R599.1[医药卫生—内科学]