Supplementation of a lacto-fermented rapeseed-seaweed blend promotes gut microbial-and gut immune-modulation in weaner piglets  

作　　者：Yan Hui Paulina Tamez-Hidalgo Tomasz Cieplak Gizaw Dabessa Satessa Witold Kot Søren Kjærulff Mette Olaf Nielsen Dennis Sandris Nielsen Lukasz Krych 

机构地区：[1]Department of Food Science,Faculty of Science,University of Copenhagen,Rolighedsvej 26,DK-1958 Frederiksberg C,Denmark [2]Fermentationexperts A/S.Vorbassevej 12,DK-6622 Baekke,Denmark [3]Department of Veterinary and Animal Sciences,Faculty of Health and Medical Sciences,University of Copenhagen,Grønnegårdsvej 3,DK-1870 Frederiksberg C,Denmark [4]Department of Plant and Environmental Sciences,Faculty of Science,University of Copenhagen,Rolighedsvej 26,DK-1958 Frederiksberg C,Denmark [5]Department of Animal Science,Faculty of Technical Sciences,Aarhus University,Blichers Alle 20,DK-8830 Tjele,Denmark

出　　处：《Journal of Animal Science and Biotechnology》2022年第1期138-151,共14页畜牧与生物技术杂志（英文版）

基　　金：financed by Bio-Based Industries Joint Undertaking under the European Union Horizon 2020 research and innovation program under grant agreement No 720755(Macro Cascade project);co-financed by the Innovation Fund Denmark and the University of Copenhagen under a PhD grant(file no.5157-00003B)for PhD study;financed by China Scholarship Council under a PhD scholarship(No.201706350028).

摘　　要：Background:The direct use of medical zinc oxide in feed will be abandoned after 2022 in Europe,leaving an urgent need for substitutes to prevent post-weaning disorders.Results:This study investigated the effect of using rapeseed-seaweed blend(rapeseed meal added two brown macroalgae species Ascophylum nodosum and Saccharina latissima)fermented by lactobacilli(FRS)as feed ingredients in piglet weaning.From d 28 of life to d 85,the piglets were fed one of three different feeding regimens(n=230 each)with inclusion of 0%,2.5% and 5% FRS.In this period,no significant difference of piglet performance was found among the three groups.From a subset of piglets(n=10 from each treatment),blood samples for hematology,biochemistry and immunoglobulin analysis,colon digesta for microbiome analysis,and jejunum and colon tissues for histopathological analyses were collected.The piglets fed with 2.5% FRS manifested alleviated intraepithelial and stromal lymphocytes infiltration in the gut,enhanced colon mucosa barrier relative to the 0% FRS group.The colon microbiota composition was determined using V3 and V1-V8 region 16S rRNA gene amplicon sequencing by Illumina NextSeq and Oxford Nanopore MinION,respectively.The two amplicon sequencing strategies showed high consistency between the detected bacteria.Both sequencing strategies indicated that inclusion of FRS reshaped the colon microbiome of weaned piglets with increased Shannon diversity.Prevotella stercorea was verified by both methods to be more abundant in the piglets supplied with FRS feed,and its abundance was positively correlated with colonic mucosa thickness but negatively correlated with blood concentrations of leucocytes and IgG.Conclusions:FRS supplementation relieved the gut lymphocyte infiltration of the weaned piglets,improved the colon mucosa barrier with altered microbiota composition.Increasing the dietary inclusion of FRS from 2.5% to 5% did not lead to further improvements.

关 键 词：Amplicon sequencing Colon microbiota Fermented feed Gut barrier 

分 类 号：S828.5[农业科学—畜牧学]