Chrysanthemum WRKY15-1 promotes resistance to Puccinia horiana Henn. via the salicylic acid signaling pathway  

作　　者：Mengmeng Bi Xueying Li Xin Yan Di Liu Ge Gao Pengfang Zhu Hongyu Mao 

机构地区：[1]College of Forestry,Shenyang Agricultural University,Shenyang 110866,China [2]Key Laboratory of Forest Tree Genetics,Breeding and Cultivation of Liaoning Province,Shenyang 110866,China

出　　处：《Horticulture Research》2021年第1期160-170,共11页园艺研究（英文）

基　　金：y the National Natural Science Foundation of China(31972447);the National Key R&D Program Projects(2018YFD1000400);the Natural Science Foundation of Liaoning Province(2019-ZD-0707).

摘　　要：Chrysanthemum white rust disease,which is caused by the fungus Puccinia horiana Henn.,severely reduces the ornamental quality and yield chrysanthemum.WRKY transcription factors function in the disease-resistance response in a variety of plants;however,it is unclear whether members of this family improve resistance to white rust disease in chrysanthemum.In this study,using PCR,we isolated a WRKY15 homologous gene,CmWRKY15-1,from the resistant chrysanthemum cultivar C029.Real-time quantitative PCR(RT-qPCR)revealed that CmWRKY15-1 exhibited differential expression patterns between the immune cultivar C029 and the susceptible cultivar Jinba upon P.horiana infection.In addition,salicylic acid(SA)treatment strongly induced CmWRKY15-1 expression.Overexpression of CmWRKY15-1 in the chrysanthemum-susceptible cultivar Jinba increased tolerance to P.horiana infection.Conversely,silencing CmWRKY15-1 via RNA interference(RNAi)in C029 increased sensitivity to P.horiana infection.We also determined that P.horiana infection increased both the endogenous SA content and the expression of salicylic acid biosynthesis genes in CmWRKY15-1-overexpressing plants,whereas CmWRKY15-1 RNAi plants exhibited the opposite effects under the same conditions.Finally,the transcript levels of pathogenesis-related(PR)genes involved in the SA pathway were positively associated with CmWRKY15-1 expression levels.Our results demonstrated that CmWRKY15-1 plays an important role in the resistance of chrysanthemum to P.horiana by influencing SA signaling.

关 键 词：expression. RESISTANCE ENDOGENOUS 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治]